{"title":"Electrophoresis of proteins of irradiated mouse chimeras.","authors":"R. Popp, L. Smith","doi":"10.1093/JNCI/23.3.395","DOIUrl":null,"url":null,"abstract":"A comparative study was made of the proteins in various tissues of Sprague-Dawley rats and (101 x C3H)F/sub 1/ mice. The results were used as a basis for studying changes in the protein patterns in tissues of rat-mouse chimeras: i.e., (101 x C3H)F/sub 1/ mice were given 950 r of x rays and then received an injection of bone marrow from Sprague-Dawley rats. Electrophoretic mobilities of rat proteins extracted from the bone marrow, spleen, lymph node, and thymus of chimeras and rats were similar. The quantity of rat proteins and the rate and time at which they appeared in different hematopoietic organs were quite different. Some mouse-type proteins not found in chimera tissues examined 30 days after treatment were frequently present on the 50th day or later, which suggests regression of the graft. The results are consistent with the idea that rat-type proteins synthesized in the cytoplasm of transplanted rat cells are controlled by the genotype of these cells, even in a mouse environment. They also indicate that there are organ-specific differences, in the rate and degree of replacement of transplanted cells, that require further investigation. (auth)","PeriodicalId":90304,"journal":{"name":"Project report. USAF School of Aviation Medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1959-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Project report. USAF School of Aviation Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/JNCI/23.3.395","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8
Abstract
A comparative study was made of the proteins in various tissues of Sprague-Dawley rats and (101 x C3H)F/sub 1/ mice. The results were used as a basis for studying changes in the protein patterns in tissues of rat-mouse chimeras: i.e., (101 x C3H)F/sub 1/ mice were given 950 r of x rays and then received an injection of bone marrow from Sprague-Dawley rats. Electrophoretic mobilities of rat proteins extracted from the bone marrow, spleen, lymph node, and thymus of chimeras and rats were similar. The quantity of rat proteins and the rate and time at which they appeared in different hematopoietic organs were quite different. Some mouse-type proteins not found in chimera tissues examined 30 days after treatment were frequently present on the 50th day or later, which suggests regression of the graft. The results are consistent with the idea that rat-type proteins synthesized in the cytoplasm of transplanted rat cells are controlled by the genotype of these cells, even in a mouse environment. They also indicate that there are organ-specific differences, in the rate and degree of replacement of transplanted cells, that require further investigation. (auth)
对Sprague-Dawley大鼠和(101 x C3H)F/sub 1/小鼠各组织中的蛋白进行了比较研究。结果作为研究大鼠-小鼠嵌合体组织中蛋白质模式变化的基础:(101 x C3H)F/sub 1/小鼠接受950 r的x射线照射,然后注射Sprague-Dawley大鼠骨髓。从嵌合体的骨髓、脾脏、淋巴结和胸腺中提取的大鼠蛋白的电泳迁移率与大鼠相似。在不同的造血器官中,大鼠蛋白的数量、出现的速率和时间都有很大的差异。在治疗后30天检查的嵌合体组织中未发现的一些小鼠型蛋白在第50天或更晚的时候经常出现,这表明移植物消退。该结果与移植大鼠细胞细胞质中合成的大鼠型蛋白受这些细胞的基因型控制的观点一致,即使在小鼠环境中也是如此。它们还表明,在移植细胞的替换率和程度上存在器官特异性差异,这需要进一步研究。(认证)