Streptolysin Encoding Genes sagC and sagD as Biomarkers of Fish Pathogen Streptococcus iniae: An In Silico Study

Q3 Environmental Science
S. Ethica, S. Darmawati, S. S. Dewi, N. Nurrahman, A. R. Sulistyaningtyas
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引用次数: 3

Abstract

Streptococcus iniae has been notorious as a serious tilapia fish pathogen leading to many disease outbreaks in warm water marine aquaculture. An in silico investigation about the potential of virulence genes of S. iniae, sagC and sagD, as biomarkers of the bacterial species, has been carried out. The aim was to determine bacterial biomarkers, which are important to facilitate early rapid diagnosis of S. iniae streptococcal infection in fish and also in humans. First, specific primers were designed from sagC and sagD genes of S. iniae SF1 genomic DNA using Primer3Plus. Next, in silico PCR (Polymerase Chain Reaction) analysis was carried out using the newly designed primers and 117 genomic DNA of streptococci (all species) retrieved from the database. Primers designed from sagC and sagD genes (SagCF: ‘5- TGCTGACCTCCTAAAAGGGC -3’ and SagCR: ‘5- CTATGCGGCGGGTTTAAGGT -3’ as well as SagDF: 5’- GCCAATCCAATCCTGTCATGC -3’ and SagDR: 5’- TGCAGCTTCCATAACCCCTC -3’) could result in a single band of each matching to 558-bp and 590-bp PCR products only from S. iniae. From 116 other streptococcal genomes studied using similar primers have resulted in no amplicon bands. A further check showed that the amplicons were truly part of sagC and sagD genes of S. iniae. These results showed that sagC and sagD genes appeared to be biomarkers of S. iniae, which are potential to be used to facilitate rapid diagnostic of the pathogenic bacterium.
溶链素编码基因sagC和sagD作为鱼致病菌海豚链球菌生物标志物的计算机研究
海豚链球菌是一种严重的罗非鱼病原体,在海水养殖中引起了许多疾病的爆发。本文对作为细菌生物标志物的葡萄球菌、sagC和sagD的毒力基因潜力进行了计算机研究。目的是确定细菌生物标志物,这对促进早期快速诊断鱼和人的猪链球菌感染很重要。首先,利用Primer3Plus软件,从金黄色葡萄球菌SF1基因组DNA的sagC和sagD基因设计特异性引物。接下来,利用新设计的引物和数据库中检索到的117个链球菌(所有种)基因组DNA进行PCR分析。sagC和sagD基因设计的引物(SagCF: ' 5- TGCTGACCTCCTAAAAGGGC -3 '和SagCR: ' 5- ctatgggcggcggggtttaaggt -3 '以及SagDF: 5 ' - gccaatcctgtcatgc -3 '和SagDR: 5 ' - TGCAGCTTCCATAACCCCTC -3 ')可以分别获得与S. iniae的558 bp和590 bp PCR产物匹配的单条带。从使用类似引物研究的116个其他链球菌基因组中,没有发现扩增子带。进一步的检测表明,扩增子确实是葡萄球菌sagC和sagD基因的一部分。这些结果表明,sagC和sagD基因可能是葡萄球菌的生物标志物,有可能用于促进病原菌的快速诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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