The Effect of Chrysin on Oxidative Stress in the Ovarian Tissue of Aspirin Administered Rats

Abstract

Aspirin is a medication with anti-inflammatory and antioxidant properties. Chrysin is a natural flavonoid that has been extensively investigated for its significant biological impacts. In this study, we aimed to investigate the protective effect of chrysin against aspirin-induced oxidative damage in rat ovarian tissue. Forty female albino rats were used in the study. Rats were randomly divided into 5 groups. Group1 (control group): No medication was administered to the rats in this group. Group 2 (ASA group): 1 mg/kg aspirin was administered to rats in this group by oral gavage for 28 days. Group 3 (ASA+CH): 1 mg/kg aspirin and 50 mg/kg CH were administered to rats in this group by oral gavage for 28 days. Group 4 (CH): 50 mg/kg CH was administered by oral gavage to the rats in this group for 28 days. Group 5 (olive oil): 1 ml/kg of olive oil was administered orally to the rats in this group. At the end of the 28-day trial, ovarian tissues were taken under anesthesia, after the rats fasted for one night. The supernatant was obtained by homogenizing the ovarian tissues, and Malondialdehyde (MDA), Advanced Oxidation Protein Products (AOPP), Total sulfhydryl (T-SH) level, and catalase (CAT) activity were quantified spectrometrically from the supernatant. Ovarian tissue MDA and AOPP levels in the ASA group were determined to be significantly higher than in the control group (p<0.05). Ovarian tissue MDA level in the ASA+CH group was lower compared to the ASA group, but the difference between the two groups was not significant (p>0.05). The AOPP level of the ASA+CH group was found to be significantly lower than that of the ASA group (p<0.05). T-SH level of the ASA group was significantly lower than the control group (p<0.05). No significant difference was determined between T-SH levels of the ASA and AS+CH group (p>0.05). CAT activity in the ASA group was lower than in the control group, but the decrease was not significant (p>0.05). Ovarian tissue CAT activity was found to be significantly higher in the ASA+CH group, compared to the control and ASA groups (p<0.05). ASA administration causes an increase in oxidative stress markers from the ovarian tissue, whereas it causes a decrease in antioxidants. By reducing this effect of aspirin, CH could increase antioxidant levels and reduce oxidative stress