Effect of powdered extract of peppermint (Mentha piperita) on adult cowpea bruchid (Callosobruchus maculatus) mortality during storage in Jos - Plateau

O. Amazu, C. Nwadike, M. Ishaya, O. Oladejo, J. Walter, Anayip Mudi, M. O. Shakirdeen, D. U. Mmadu
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Abstract

The over dependence and continues use of synthetic insecticides to control storage pests have been associated with several demerits. This study was conducted to evaluate the insecticidal effect of peppermint (Mentha piperita) leaf powder on Callosobruchus maculatus. The peppermint plant was sourced locally and the C. maculatus were cultured to obtain the adult Bruchids. The peppermint was washed, dried under ambient temperature and pulverized. Complete Randomized Design was adopted for this trial with three replications of six treatment dosages of 2.5, 5.0, 7.5, 10.0, 12.5 and 0.0 g respectively of the plant material. The trial lasted for four weeks. High Bruchids mortality was observed after treatment with Mentha piperita leaf powder extract. Phytochemical screening of the test plant revealed presence of flavonoids, tannins, saponins, carbohydrates and cardiac glycosides. Descriptive statistic and analysis of variance (ANOVA) were used to analyze the data. Means (p≤0.05) were separated using Duncan Multiple Range Test (DMRT). Dosage rate and mean mortality values at the various doses were statistically significant after 48, 72 and 96 hours. The mortality could be attributed to high presence flavonoid in the Mentha piperita leaf powder. The results suggest that the leaf of peppermint has insecticidal property against adult C. maculatus. However, further trials are required to investigate effective dosage rates for other stored grains and to isolate and characterize the active flavonoids contained in the plant.
薄荷粉末提取物对乔斯高原贮存期豇豆成虫死亡率的影响
过度依赖和持续使用合成杀虫剂来控制储藏害虫有几个缺点。本试验研究了薄荷叶粉对斑孔雀鱼的杀虫效果。以当地薄荷植物为原料,培养成薄荷。薄荷洗净,在常温下干燥,粉碎。试验采用完全随机设计,3个重复,分别为2.5、5.0、7.5、10.0、12.5、0.0 g 6个处理剂量。审判持续了四周。薄荷叶粉提取物处理后,野鼠死亡率较高。植物化学筛选结果显示,实验植物中含有黄酮类化合物、单宁、皂苷、碳水化合物和心苷。采用描述性统计和方差分析(ANOVA)对数据进行分析。采用Duncan多元极差检验(DMRT)分离均数(p≤0.05)。48小时、72小时和96小时后,不同剂量的给药率和平均死亡率均有统计学意义。其死亡可能与薄荷叶粉中黄酮类化合物含量高有关。结果表明,薄荷叶对成虫有杀虫作用。然而,需要进一步的试验来研究其他储存谷物的有效剂量率,并分离和表征植物中含有的活性黄酮类化合物。
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