Size Exclusion Chromatography of Poly(ethylene glycol)

Abstract

Size exclusion chromatography calibrations for polystyrene and poly(ethylene glycol) standard samples in the molecular weight range of (600-11000) have been obtained in tetrahydrofuran. The Kuhn-Mark-Houwink-Sakurada constants of poly(ethylene glycol) in tetrahydrofuran were determined, and poly(ethylene glycol) chromatograms were evaluated according to primary and universal calibration methods. The molecular weights determined from universal curves were substantially lower than those obtained from primary curves. The Coll and Prusinowski universal calibration procedure gave better results than the calibration of Benoit et al.