Methylation levels of lysozyme gene in rainbow trout (Oncorhynchus mykiss) fed by commercial immunogen probiotic

Abstract

In the present study, we evaluated the effect of commercial immunogenic prebiotic on the rate of methylation of type-C gene. A total of 120 rainbow trout were divided into two treatments, including a control group and another one with 2.0% commercial prebiotic immunogen each in three replicates. On the first, 15 and 45 days, DNA of adrenal tissue was extracted and treated with bisulfite. Samples were amplified by polymerase chain reaction (PCR) and sequenced. Based on the results, there was no significant difference ( P <0.05) between the 1st day (R), 15 th day of control (C), 15 th day of immunogen (I) and 40 th day of control (CS). However, there was a significant difference ( P <0.05) between the 45 th day immunogenicity (IS) and other samples, i.e. the CpG islet methylation rate in the IS samples was lower leading to increase in the expression of the lysozyme gene