Effect of fluoride on expression of runx2 mRNA and protein in bone tissue of rats

Q3 Medicine

中华地方病学杂志 Pub Date : 2010-09-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2010.05.007

M. Mei, Yu Yan-ni, Guo Bing

引用次数: 0

Abstract

Objective To investigate the effect of fluoride on expression of Runx2 mRNA and protein in bone tissue of rats. Methods Fourteen SD rats were randomly divided into two groups: control group(tap water with fluoride ＜ 0.06 mg/L), and fluorosis group(fluoride 50 mg/L in water). After 4 moths, expressions of both mRNA and protein of Runx2 in rat bone tissue were determined by RT-PCR and Western blotting. Results The results showed that the expression of Runx2 mRNA and protein in fluoride-treated bone tissue were 2.287 ± 0.261 and 0.929 ± 0.229, respectively, both of which were significantly higher than those of control group(0.995 ± 0.123,0.317 ± 0.068, t = 11.85,6.78, P ＜ 0.05). Conclusions Fluoride can increase the expression of Runx2 mRNA and protein in bone tissue of rats, and Runx2 may be involved in the pathogenesis of bone injury caused by fluoride. Key words: Fluorides; Genes; Proteins; Runx2

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氟对大鼠骨组织runx2 mRNA及蛋白表达的影响

目的探讨氟对大鼠骨组织Runx2 mRNA及蛋白表达的影响。方法将14只SD大鼠随机分为两组:对照组(自来水中氟化物< 0.06 mg/L)和氟中毒组(水中氟化物50 mg/L)。4个月后，采用RT-PCR和Western blotting检测Runx2 mRNA和蛋白在大鼠骨组织中的表达。结果氟化骨组织中Runx2 mRNA和蛋白的表达量分别为2.287±0.261和0.929±0.229，均显著高于对照组(0.995±0.123、0.317±0.068,t = 11.85、6.78,P < 0.05)。结论氟可增加大鼠骨组织中Runx2 mRNA和蛋白的表达，Runx2可能参与了氟致骨损伤的发病机制。关键词:氟化物;基因;蛋白质;Runx2

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.