Genetic diversity of sessile oak populations in the Czech Republic

Abstract

The sessile oak is a broadleaved tree species of great ecological and silvicultural importance. Oaks are the second most widespread deciduous tree species in the Czech Republic, and ongoing climate change negatively affects the abundant and often monocultural Norway spruce. Therefore, a proportional increase of more resilient tree species such as sessile oak has emerged. This study aimed to depict population genetic diversity when analysing 272 individuals from 10 subpopulations selected across the Czech Republic. Targeted populations were chosen based on the minimal expected human impact on the stand (presumably autochthonous stands). All individuals were genotyped using 18 polymorphic microsatellite markers (SSRs) assembled into two amplification multiplexes. The high discriminatory power of SSR markers was tested and confirmed by the probability of identity analysis. The genetic differentiation of the subpopulations was low yet significant, quantified by Wright’s F-statistics within the range from 0.012 to 0.029. Based on discriminant analysis of principal components (DAPC), we detected two populations with geographic genetic correlation (the 15th meridian east being a north-south boundary line) and one with a distinct genetic pattern. We assume that the population might previously be established from seed sources outside the Czech Republic. Moreover, to some extent, our findings advocate the legitimacy of the legislative rules for forest reproductive material (FRM) transfer.