Molecular phylogeny of four ascidian species inferred from mitochondrial Cytochrome Oxidase subunit I (COI) sequence

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY

Mitochondrial Dna Part a Pub Date : 2018-01-02 DOI:10.1080/24701394.2016.1253071

G. Ananthan, R. Murugan

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引用次数: 6

Abstract

Abstract Ascidians is a crucial group for the studies of deuterostome evolution and the origin of chordates, yet little molecular work has been done to determine the evolutionary relationships and largely unexplored beyond a few species. The phylogenetic analysis are presented for four different species of solitary ascidians, Ascidia virginea, Ascidiella aspersa, Clavelina oblonga and Aplidium fuscum, obtained from Great Nicobar Biosphere reserve (GNBR). Mitochondrial Cytochrome Oxidase subunit 1(COI) gene was amplified and the genetic diversity at the phylogenic level was measured. Bar-coded sequences were extracted with BLAST format from NCBI and the genetic diversity of the submitted sequences were compared with the related ascidian species. Maximum divergences measured among the four species were as follows: Ascidia virginea (96%), Ascidiella aspersa (96%), Clavelina oblonga (94%) and Aplidium fuscum (97%). This is the first report of molecular phylogeny of ascidians from Great Nicobar Biosphere Reserve, Andaman and Nicobar Islands. From this study, we found some stable clades on the evolutionary relationships among these ascidian species that may prompt a reevaluation of some morphological characters.

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从线粒体细胞色素氧化酶亚单位I (COI)序列推断的四种海鞘的分子系统发育

摘要海鞘是研究后口动物进化和脊索动物起源的重要类群，但其进化关系的分子研究很少，而且除了少数物种外，还未得到充分的探索。本文对大Nicobar生物圈保护区(GNBR) 4种不同的独居海鞘(Ascidia virginea、Ascidiella aspersa、clvelina oblonga和Aplidium fuscum)进行了系统发育分析。扩增线粒体细胞色素氧化酶亚基1(COI)基因，并在系统发育水平上测定遗传多样性。利用BLAST格式从NCBI中提取条形码序列，并与相关海鞘种的遗传多样性进行比较。4种间差异最大的分别为:virginedia(96%)、aspersa Ascidiella(96%)、clvelina oblonga(94%)和Aplidium fuscum(97%)。本文首次报道了安达曼和尼科巴群岛大尼科巴生物圈保护区海鞘的分子系统发育。通过本研究，我们发现了一些稳定的海鞘进化关系分支，这可能会促使我们重新评估一些形态特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.