Avian intrapulmonary chemoreceptor discharge rate is increased by anion exchange blocker ‘DIDS’

J.M Shoemaker , S.C Hempleman
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引用次数: 17

Abstract

Avian intrapulmonary chemoreceptors (IPC) are neurons that sense lung PCO2 and provide phasic feedback for the control of breathing in birds. To try to understand mechanisms of CO2 transduction and intracellular pH regulation in IPC, the anion exchange inhibitor 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) was used to block transmembrane Cl/HCO3 transport. Single-unit IPC discharge rates were measured at steady intrapulmonary CO2 levels and during step changes in CO2 in 15 anesthetized, unidirectionally ventilated adult mallard ducks (Anas platyrhynchos). Measurements were repeated after giving 50, 100 and 200 μmol/kg cumulative i.v. dosages of DIDS. Mean IPC discharge rates at steady (tonic) PCO2 levels were significantly increased by 100 and 200 μmol/kg DIDS, but not by 50 μmol/kg DIDS. Mean dynamic (phasic) IPC responses to CO2 steps were not significantly affected by DIDS. Results indicate that the DIDS-sensitive Cl/HCO3 membrane exchanger is involved with tonic CO2 signal transduction in IPC. However, because some individual IPC were unaffected by DIDS, yet still altered their discharge rate with CO2, additional mechanisms besides the Cl/HCO3 exchange are probably required for CO2 chemotransduction in IPC.

阴离子交换阻滞剂DIDS增加禽类肺内化学受体排出率
鸟类肺内化学感受器(IPC)是一种感知肺部二氧化碳分压并为鸟类呼吸控制提供相位反馈的神经元。为了了解IPC中CO2转导和细胞内pH调节的机制,使用阴离子交换抑制剂4,4 ' -二异硫氰二苯乙烯-2,2 ' -二磺酸(DIDS)阻断Cl - /HCO3 -跨膜转运。对15只麻醉、单向通气的成年绿头鸭(Anas platyrhynchos)在稳定的肺内CO2水平和CO2阶跃变化期间的单单位IPC放电率进行了测量。分别给药50、100和200 μmol/kg后重复测量。在稳定(tonic) PCO2水平下,平均IPC排放率增加了100和200 μmol/kg DIDS,但没有增加50 μmol/kg DIDS。平均动态(相位)IPC对CO2阶跃的响应不受DIDS的显著影响。结果表明,dids敏感的Cl−/HCO3−膜交换器参与了IPC中的强直CO2信号转导。然而,由于一些个体IPC不受DIDS的影响,但仍然改变了它们对CO2的排放速率,因此除了Cl - /HCO3 -交换之外,IPC中的CO2化学转导可能还需要其他机制。
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