Resveratrol pretreatment reduces circulating inflammatory interleukins in CCl4-induced hepatotoxicity rats

Abstract

Liver diseases represent one of major health burdens worldwide, both oxidative stress and inflammation play crucial roles in the development of liver diseases. Resveratrol (RSV), a naturally occurring compound, has recently been shown to exert anti-oxidative and anti-inflammatory actions. This work aimed to investigate the prophylactic effect of RSV against CCl₄-induced hepatotoxicity in experimental rats in terms of inflammatory interleukins and histopathological changes. Twenty-four adult male rats were divided into three equal groups: group A: Negative control, received oral vehicle, group B: Positive control, received oral vehicle for 6 days and on 6^th day injected with single dose of CCl₄, and group C: RSV-treated group, received oral RSV (25 mg/kg/day) for 6 days and on 6^th day injected with single dose of CCl₄. 24 h after induction of hepatotoxicity by CCl₄, all rats were sacrificed; liver was excised for histopathological studies and blood samples were collected. ALT, AST, ALP, total bilirubin, IL-1β, IL-6, and IL-10 were determined in plasma while total GSH, MDA, GPx, and SOD were assayed in liver homogenate. Plasma levels of ALT, AST, ALP, total-bilirubin, IL-1β, and IL-6 were significantly increased 24 h after induction of hepatotoxicity by CCl₄ while IL-10 decreased. Pretreatment of rats with RSV prevented these changes. The histopathological changes were less obvious in livers of RSV-treated rats in comparative to positive control. In conclusion, RSV has a prophylactic effect against hepatotoxicity induced by CCl₄ through decreasing the inflammatory interleukins level.