Potentiometric determination of organic acids in the medicinal plant raw material

T. Oproshanska, O. Khvorost
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引用次数: 4

Abstract

Aim. To analyze the results of the quantitative content of the amount of organic acids in rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite by the potentiometric method, which is available in the monograph “Hibiscus” in the SPhU 2.0. Materials and methods. The study subjects were batches of rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite. The quantitative content of the amount of organic acids was determined by the potentiometric method from the monograph “Hibiscus” SPhU 2.0 using a HI 2550 potentiometer of the “HANNA instruments” company (Germany). Results and discussion. The highest content of the amount of organic acids, among the study subjects was in roots of Arctium minus (from 1.02 ± 0.04 % to 1.05 ± 0.04 %). At the same time, in roots of Arctium tomentosum this amount was almost 3 times lower (from 0.36 ± 0.01 % to 0.37 ± 0.01 %). In the batches of roots of Rosa majalis the content of this group of compounds was almost 2 times higher than in roots of Rosa canina. In rhizomes with roots of Rumex confertus and rhizomes with roots of Sanguisorba officinalis the content of the amount of organic acids differed insignificantly. In herb of Bidens tripartite the content of this group of compounds was 0.78 ± 0.03  – 0.79 ± 0.03 %. In different batches of one type of the raw material the quantitative content of the amount of organic acids varied slightly. Conclusions. For the first time, the quantitative content of the amount of organic acids has been determined in the batches of rhizomes with roots of Rumex confertus, rhizomes with roots of Sanguisorba officinalis, roots of Rosa majalis and roots of Rosa canina, roots of Arctium minus and roots of Arctium tomentosum, herb of Bidens tripartite using the potentiometric method which is available in the monograph “Hibiscus” in the SPhU 2.0. It has been found that the highest content of the amount of organic acids is observed in roots of Arctium minus (not less than 1.02 %) and herb of Bidens tripartite (not less than 0.78 %). The results obtained will be used in further research when creating new herbal medicines with the anti-inflammatory and antimicrobial activity.
药用植物原料中有机酸的电位法测定
的目标。采用电位法对SPhU 2.0中木槿专著《木槿》中胡芦巴根茎、血地榆根茎、马茱萸根茎和狗尾花根茎、牛蒡根和毛蕊牛蒡根、三连草中有机酸含量的定量测定结果进行分析。材料和方法。研究对象为成批的牛尾藤根茎、血地榆根茎、马茱萸根茎和犬牙蔷薇根、牛蒡根和毛蕊牛蒡根、三棱草草本植物。有机酸的定量含量采用德国“HANNA instruments”公司的HI 2550电位器,采用电位法,取自《Hibiscus》SPhU 2.0专著。结果和讨论。有机酸含量最高的是牛蒡根(1.02±0.04% ~ 1.05±0.04%)。与此同时,牛蒡根的这一含量几乎降低了3倍(从0.36±0.01%降至0.37±0.01%)。在不同批次的红蔷薇根中,这类化合物的含量几乎是红蔷薇根的2倍。山楂根茎和地榆根茎中有机酸含量差异不显著。在三叶草中,这类化合物的含量为0.78±0.03 ~ 0.79±0.03%。同一种原料在不同批次中有机酸的定量含量略有差异。本文首次采用SPhU 2.0《木槿》专著中所载的电位法,定量测定了红毛藤根茎、血地榆根茎、马茱萸根茎和犬玫瑰根茎、牛蒡根和毛蕊牛蒡根、木槿三叶草等药材的有机酸含量。有机酸含量最高的是牛蒡根(不低于1.02%)和菟丝子根(不低于0.78%)。所得结果将用于进一步研究开发具有抗炎和抗菌活性的新型草药。
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