Hepato therapeutic Efficacy of Native Curcumim and Nano –curcumin : A Novel Therapy Against Hyperthyroidism Induced Liver Oxidative and Inflammatory Damage in Rats

Abstract

Recent works have indicated the beneficial efficacy of using nanocurcumin in addressing poor bioavailability commonly associated with native curcumin. Curcumin has been shown to alleviate hyperthyroidism induced liver dysfunction. Here we carried out a comparative study using both native curcumin and nanocurcumin to verify the superior effects of encapsulation of curcumin in nanomaterials in blunting liver dysfunction in L-thyroxine induced hyperthyroid rats. Native curcumin (NC) and poly (lactide-co-glycolic acid (PLGA) encapsulated curcumin (PNC) significantly increased liver catalase activity and reduced glutathione (GSH) levels and significantly declined malondialdehyde (MDA) levels in hyperthyroid rats. However, rats treated with PNC had significantly better ameliorative effects on these parameters than rats treated with NC. Similarly, PNC showed significantly improved effects in lowering inflammatory mediators including TNF-a, IL-6, VEGF, CRP and caspase protein levels compared to NC in hyperthyroid rats. Serum T3, T4, and TSH levels and liver functional markers including albumin, ALT, ASP and AST were better controlled in PNC treated rats than in NC treated hyperthyroid rats. DNA fragmentation as estimated by comet assay was significantly lower in PNC treated rats than in NC treated rats. Likewise, significantly fewer histopathological and ultrastructural changes were observed in liver tissue in PNC treated hyperthyroid rats. Conclusion: Our work illustrates the capability of PNC to resolve hyperthyroidism induced liver dysfunction and substantiate the findings that beneficial effects of curcumin may be maximized by improving its stability and bioavailability by its encapsulation in nanoparticles.