Antimicrobial activities of some medicinal plant extracts based on nanobiomedicine

R. Gashgari, Abrar Othman
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Abstract

The evaluation of antimicrobial study of few medicinally used plants to control the growth of some pathogenic microorganisms. The standard protocols with slight modifications were utilized to prepare silver nanoparticles using five medicinal plants extracts namely, Juniperus exclsa, Moringa oleifera, Prosopis juliflora, Pithecellobium dulce and Eucalyptus globulus, were randomly collected from Jeddah and Taif city, Kingdom of Saudi Arabia. The formation of nanoparticles was observed by UV/Vis spectra and further characterization has been carried out by different analytical techniques. The shape, size and morphology of nanoparticles were observed by SEM and TEM images. The prepared extracts and nanoparticles were tested for antimicrobial study and the total phenolics of the methanolic extract was confirmed by GCMS and HPLC techniques. The nanoparticles were assessed against five microorganisms such as Gram-positive Staphylococcus aureus ATCC 6538, Gram-negative Pseudomonas aeruginosa ATCC 9027 and Escherichia coli ATCC 8739, yeast Candida albicans ATCC 10231and fungi Aspergillus niger ATCC 16404.
基于纳米生物医学的几种药用植物提取物的抗菌活性研究
几种药用植物对控制某些病原微生物生长的抗菌研究评价。采用在沙特阿拉伯王国吉达和塔伊夫市随机采集的五种药用植物提取物,即朱柏(Juniperus)、辣木(Moringa oleifera)、毛豆(Prosopis juliflora)、细叶桉(Pithecellobium dulce)和蓝桉(Eucalyptus globulus),利用稍加修改的标准方案制备纳米银。通过紫外/可见光谱观察到纳米颗粒的形成,并通过不同的分析技术进行了进一步的表征。通过扫描电镜(SEM)和透射电镜(TEM)观察纳米颗粒的形状、大小和形貌。对制备的提取物和纳米颗粒进行了抗菌研究,并通过gc - cms和HPLC技术对甲醇提取物的总酚类物质进行了测定。研究了纳米颗粒对革兰氏阳性金黄色葡萄球菌ATCC 6538、革兰氏阴性铜绿假单胞菌ATCC 9027和大肠杆菌ATCC 8739、白色念珠菌ATCC 10231和真菌黑曲霉ATCC 16404等5种微生物的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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