Effect of Lead and γ-Polyglutamic Acid Produced from Bacillus subtilis on Growth of Brassica chinensis L.

APCBEE Procedia Pub Date : 2014-01-01 DOI:10.1016/j.apcbee.2014.10.051

O. Chunhachart , N. Kotabin , N. Yadee , Y. Tahara , K. Issakul

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引用次数: 6

Abstract

γ-Polyglutamic acid (γ-PGA) has been reported to be an effective biosorbent for metal ions. In vitro binding of lead (II) (Pb) by γ-PGA produced from Bacillus subtilis NBRC16449 was examined and the effect of Pb and γ-PGA on Brassica chinensis L. grown in the laboratory was investigated. The results revealed that the optimal pH for Pb adsorption was 5.0. γ-PGA was stable at 35-50 °C and pH 5-7. The maximum removal efficiency was 87.9%. Brassica chinensis L. seeds were germinated and grown at 28±1 ^oC on filter paper soaked with Pb solution at 0, 50, 100 and 250 μM and γ-PGA at 0, 100, 500 and 1,000 mg/L for 7 days. The results indicated that Pb markedly inhibited growth of roots by reducing root length (P<0.05). However; the addition of 500 mg/L γ-PGA significantly protected seedlings from the adverse effects of Pb (P<0.05). Thus, γ-PGA has high potential as a biopolymer to be used for alleviation of Pb toxicities in plants.

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枯草芽孢杆菌产铅和γ-聚谷氨酸对芸苔生长的影响。

γ-聚谷氨酸(γ-PGA)是一种有效的金属离子生物吸附剂。研究了枯草芽孢杆菌NBRC16449产生的γ-PGA对铅(II) (Pb)的体外结合作用，并研究了Pb和γ-PGA对室内栽培的芸苔的影响。结果表明，吸附Pb的最佳pH为5.0。γ-PGA在35-50℃、pH 5-7条件下稳定。最高去除率为87.9%。在28±1℃条件下，在含铅浓度为0、50、100和250 μM、γ-PGA浓度为0、100、500和1000 mg/L的滤纸上萌发和生长7 d。结果表明，Pb通过减少根长显著抑制根系生长(P<0.05)。然而;添加500 mg/L γ-PGA显著保护幼苗免受Pb的不良影响(P<0.05)。因此，γ-PGA作为一种生物聚合物具有很大的潜力，可用于减轻植物的铅毒性。

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APCBEE Procedia

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