COMPARATIVE ANALYSIS OF 16SrRNA GENES OF Klebsiella ISOLATED FROM GROUNDNUT AND SOME AMERICAN TYPE CULTURE COLLECTIONS

Abstract

Endophytic and associative bacteria like Klebsiella are known to enhance growth and yield of plants by fixing atmospheric nitrogen, solubilization of phosphate, production of phytohormones and siderophores. Despite this, less genetic information known concerning this important bacteria. Thus, this study was conducted to explore the relationship between Klebsiella spp. isolated from groundnut from Gadarif (Sudan) and some American type culture collections through 16SrRNA gene analysis. Gadarif strain was isolated using Yeast Extract Mannitol Media, DNA was extracted, 16SrRNA gene was amplified and sequenced. The results showed that Klebsiella pneumoniae from the American type culture collections was the most related to that isolated from Gadarif and Klebsiella oxytoca was the most different. The majority differences were found at several distinct positions. The study concluded that Klebsiella spp. isolated from different regions and different environmental conditions differ in their DNA sequences. Keyword: Endophytic, groundnut, isolate, nucleotides, sequences.