Application of Conventional Techniques for the Diagnosis of Staphylococcus lugdunensis in Clinical Microbiology

L. López, Milagros de la Caridad Milá Pascual, José Antúnez Coca
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Abstract

Introduction: the participation of coagulase negative staphylococci in human diseases is an important problem, particularly in hospital settings where these species often act as opportunistic pathogens. Objective: to show the results obtained after applying conventional techniques for its detection. Results: the tests Cumpling factor, Detection of hemolysin δ-like, acidification of the substrates trehalose, mannose, mannitol and inositol were applied, as well as the ornithine decarboxylation biochemical test and the behavior of antimicrobial sensitivity was taken into account. all being feasible for the diagnosis of this microorganism due to the results obtained. Conclusions: Staphylococcus lugdunensis is an atypical SCoN compared to the rest of the SCoNs, both due to its special virulence characteristics and its striking sensitivity to multiple antimicrobials, including penicillin sensitivity in many cases. Its diagnosis in clinical microbiology laboratories is simple, given the suspicion of this species by the positive compliance factor test, a complete identification of it must be carried out through the decarboxylation of ornithine, the acidification of mannose, as well as antimicrobial sensitivity, are useful tests to differentiate this microorganism from the rest of its species.
常规技术在临床微生物学诊断中的应用
导言:凝固酶阴性葡萄球菌在人类疾病中的参与是一个重要的问题,特别是在医院环境中,这些物种经常作为机会性病原体。目的:展示常规检测方法对其检测结果。结果:采用复合因子试验、溶血素δ样检测、底物海藻糖、甘露糖、甘露醇和肌醇酸化试验,以及鸟氨酸脱羧生化试验和抗菌敏感性行为。结果表明,对该微生物的诊断是可行的。结论:与其他SCoN相比,lugdunensis葡萄球菌是一种非典型的SCoN,这既是由于其特殊的毒力特征,也是由于其对多种抗菌素的惊人敏感性,包括在许多情况下对青霉素的敏感性。临床微生物实验室对其的诊断很简单,考虑到通过阳性依从性因子试验对该物种的怀疑,必须通过鸟氨酸的脱羧、甘露糖的酸化以及抗菌敏感性来进行完整的鉴定,这些都是将该微生物与其他物种区分开来的有用试验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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