The Effect of Brassica napus Biorenovated Extract on the Production of Melanin Production and Tyrosinase Activity in B16F10 Melanoma Cells

Abstract

Brassica napus (BN) has been reported to have various drug efficacies such as cancer, aging, but there is research on the scientific efficacy of whitening effect. Biorenovation is a method of modifying the structure of a broad range of substrates such as chemical compounds and plant extract by microbial enzymes with the potential benefits of reduced cytotoxicities and enhanced biological activities relative to its parent substrates. we generated BN extract using biorenovation technology and tested the whitening effect properties of its biorenovation product (BNBR) in α-MSH stimulated B16F10 melanoma cells. Our results showed that BNBR significantly inhibited enzymes of melanogenesis such as TRP-1, TRP-2, MITF, tyrosinase in a concentration dependent manner without no noticeable toxicities. Therefore, it suggests that maybe various components that converted by biorenovation contained in the BNBR affect multiple factors involved in α-MSH-induced melanogenesis in B16F10 cells. And our results indicate that BNBR could be a new source for the ingredients of functional cosmetics.