Study the Haematological parameters and Oxidative stress before and after disbudding in Calves

Journal of Veterinary Science and Research Pub Date : 2021-11-17 DOI:10.36811/jvsr.2021.110018

Shiv Swaroop, Pramanik PS, Singh KD, Subodh Kumar, Verma AK

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Abstract

The aim of the present investigation was to examine the haematological profile and oxidative stress before and after disbudding in calves. The study was carried out at Livestock Farm Complex, College of Veterinary Science and Animal Husbandry, ANDUAT, Kumarganj, Ayodhya and nearby villages. Twenty four cattle calves were utilized to study haematological parameters that are Haemoglobin (Hb (gm/dl), Neutrophil (%), Basophil (%), PCV (%), Lymphocyte (%), Monocyte (%), TLC (103/ µl), Eosinophil (%), N/L ratio) and oxidative stress before and after disbudding in to four groups viz. control (T0), lignocaine (TL), tremadol (TT) and lignocaine+tremadol (TL-T). After medication calves were disbudded by hot iron method. The blood samples were collected for haematological parameters & for estimation of LPO (lipid peroxides) as per method described by [1] just before (0h) and after disbudding on 6h, 24h and 72h. The results showed that, at 24h, T0 group showed significantly lowest Hb (10.66±0.49%) which was statistically similar to TT and TL-T groups. At 0h, 6h and 72h means of PCV% were significantly different among treatment groups. The lowest lymphocytes (64.00±1.00) was observed in TT group at 6h. The T0 group showed significantly lowest basophil (0.00±0.00) which was statistically similar to TT and TL-T groups but significantly different from TL group at 6h. At the same interval, T0 group showed significantly lowest monocytes (4.66±0.61) which was significantly different with TL, TT and TL-T groups. At 6h, T0 group showed significantly lowest N/ L ratio (0.15±0.01) which was statistically similar to TT and TL-T groups but significantly different from TL group. At 0h, 6h and 24h LPO was significantly different among groups. At 0h, T0 group showed significantly highest (106.59±0.63) which was significantly different from TL, TT and TL-T groups. It may be concluded that no significant difference was observed in eosinophil%, TLC, neutrophil% and monocyte% counts between control and treatment groups. However, Hb%, basophil%, PCV% and lymphocyte% were significantly different between control and treatment groups. N/L ratio was significant only at 6h. Most of the cases TL-T group showed comparatively better performance. At 0h, 6h and 24h LPO was significantly different among groups. At 0h, T0 group showed significantly highest (106.59±0.63) which was significantly different from TL, TT and TL-T groups.

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研究犊牛脱芽前后的血液学参数和氧化应激

本研究的目的是研究小牛脱芽前后的血液学特征和氧化应激。该研究是在畜牧综合农场、兽医科学与畜牧学院、ANDUAT、Kumarganj、Ayodhya和附近村庄进行的。采用24头牛，分为对照组(T0)、利多卡因(TL)、曲马多酚(TT)和利多卡因+曲马多酚(TL- t) 4组，研究脱模前后血红蛋白(Hb (gm/dl)、中性粒细胞(%)、嗜碱性粒细胞(%)、PCV(%)、淋巴细胞(%)、单核细胞(%)、薄层色谱(103/µl)、嗜酸性粒细胞(%)、N/ l比值)和氧化应激等血液学参数。用药后用热铁法对犊牛进行脱毛。在脱光前(0h)和脱光后(6h、24h和72h)，按照[1]描述的方法采集血液样本用于血液学参数和LPO(脂质过氧化物)的估计。结果显示，在24h, T0组Hb最低(10.66±0.49%)，与TT和TL-T组差异有统计学意义。0h、6h、72h PCV%的平均值各组间差异有统计学意义。TT组6h淋巴细胞最低(64.00±1.00)。T0组6h嗜碱性粒细胞最低(0.00±0.00)，与TT、TL- t组比较差异有统计学意义，但与TL组比较差异有统计学意义。同一时间内，T0组单核细胞数最低(4.66±0.61)，与TL、TT、TL- t组差异有统计学意义。6h时，T0组N/ L比最低(0.15±0.01)，与TT、TL- t组差异有统计学意义，与TL组差异有统计学意义。0h、6h、24h LPO组间差异有统计学意义。0h时，T0组最高(106.59±0.63)，与TL、TT和TL- t组差异有统计学意义。由此可见，对照组和治疗组在嗜酸性粒细胞%、TLC、中性粒细胞%和单核细胞%计数上均无显著差异。对照组与治疗组Hb%、嗜碱性粒细胞%、PCV%、淋巴细胞%差异有统计学意义。N/L比值仅在6h显著。TL-T组多数病例表现较好。0h、6h、24h LPO组间差异有统计学意义。0h时，T0组最高(106.59±0.63)，与TL、TT和TL- t组差异有统计学意义。

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Journal of Veterinary Science and Research

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