In-vitro Cytotoxicity and Antioxidant Studies of Elaeocarpus serratus

Stamford Journal of Pharmaceutical Sciences Pub Date : 2010-08-12 DOI:10.3329/SJPS.V2I2.5829

Most. Nazma Parvin, Shammy Sarwar, S. Chowdhury, H. Zakaria, N. Huda

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引用次数: 5

Abstract

The pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract of stem bark of Elaeocarpus serratus were investigated for cytotoxic activity using brine shrimp lethality bioassay .The methanolic extract was also evaluated for possible antioxidant activity using nitric oxide scavenging activity and reducing power assays. In Elaeocarpus serratus , the LC 50 value were found to be 14.94, 0.831 & 3.288 μg/mL in pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract respectively. The extract showed significant antioxidant activity in nitric oxide scavenging activity and reducing power assay. In nitric oxide scavenging activity, the IC 50 value of Elaeocarpus serratus extract was 89.325μg/mL while the IC 50 value of ascorbic acid was 47.684μg/mL. It was found that scavenging of nitric oxide by the extract was concentration dependent. Key words: Elaeocarpus serratus ; Cytotoxicity; Antioxidant; Reducing power. DOI: 10.3329/sjps.v2i2.5829 Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 86-90

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锯柏的体外细胞毒性和抗氧化研究

采用盐水对虾致死性生物测定法研究了菖蒲茎皮甲醇提取物的pet-醚溶出部分(PEF)、四氯化碳溶出部分(CTSF)和氯仿溶出部分(CFSF)的细胞毒活性，并通过一氧化氮清除活性和还原力测定法评价了甲醇提取物的抗氧化活性。松柏甲醇提取物的pet-醚溶性部位(PEF)、四氯化碳溶性部位(CTSF)和氯仿溶性部位(CFSF)的LC 50值分别为14.94、0.831和3.288 μg/mL。提取物对一氧化氮的清除活性和还原力均有显著的抗氧化活性。在一氧化氮清除活性方面，菖菖树提取物的ic50值为89.325μg/mL，抗坏血酸的ic50值为47.684μg/mL。结果表明，该提取物对一氧化氮的清除作用具有浓度依赖性。关键词:锯腕肌;细胞毒性;抗氧化剂;还原能力。DOI: 10.3329/sjps.v2i2.5829斯坦福药物科学杂志Vol.2(2) 2009: 86-90

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Stamford Journal of Pharmaceutical Sciences

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