Study of total antioxidant capacity of red raspberry (Rubus idaeous L.) shoots

Q3 Pharmacology, Toxicology and Pharmaceutics

Vitae Pub Date : 2023-04-22 DOI:10.17533/udea.vitae.v30n1a351486

O. Maslov, M. Komisarenko, M. Golik, S. Kolisnyk, Alexander A. Altukhov, S. V. Baiurka, S. Karpushina, O. Tkachenko, Kolisnyk Iuliia

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引用次数: 0

Abstract

BACKGROUND: Today, cardiovascular, oncological, and neurodegenerative diseases are the main causes of death in the world, according to official World Health Organization (WHO) statistics. Antioxidants are used to treat and prevent these diseases. In order to develop optimal technology for obtaining drugs based on plant extracts with antioxidant action, it is necessary to determine the total antioxidant capacity of raspberry shoots.OBJECTIVES: The study aimed to determine the total antioxidant capacity of red raspberry shoots, study the content of biologically active substances (BAS), and the antioxidant activity of red raspberry shoot extracts obtained during subsequent exhaustive extraction.METHODS: The number of phenolic compounds, catechins, flavonoids, and hydroxycinnamic acids was determined by a spectrophotometric analysis method, whereas organic acids were determined by the alkalimetric method in red raspberry shoot extracts; the antioxidant activity of obtained extracts was evaluated by potentiometric method. RESULTS: The total antioxidant capacity of red raspberry shoots was 164.12 mmol-equiv./m dry weight, the sum of the total content of phenolic compounds was 24.40 mg gallic acid (GA)/mL, catechins – 21.36 mg epigallocatechin-3-O-gallate (EGCG)/mL, flavonoids – 0.77 mg rutin (R)/mL, hydroxycinnamic acids derivatives – 2.56 mg chlorogenic acid (ChA)/mL and organic acids – 1.88 mg citric acid (CA)/mL in red raspberry shoot extracts obtained during subsequent exhaustive extraction. The analysis showed that there is a very high positive correlation between antioxidant activity and total phenolic compounds, catechin, flavonoid, hydroxycinnamic acids derivatives, and organic acids content in red raspberry shoot extracts. CONCLUSIONS: Total red raspberry shoots' antioxidant capacity has been determined. The study results can be used to develop optimal technology for obtaining drugs based on the extract of red raspberry shoots, which has an antioxidant effect.

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红覆盆子芽总抗氧化能力的研究

背景:根据世界卫生组织(WHO)的官方统计，今天，心血管、肿瘤和神经退行性疾病是世界上死亡的主要原因。抗氧化剂被用来治疗和预防这些疾病。为了开发具有抗氧化作用的植物提取物制备药物的最佳工艺，有必要对覆盆子芽的总抗氧化能力进行测定。目的:测定红覆盆子芽的总抗氧化能力，研究其生物活性物质(BAS)的含量，以及随后穷尽提取得到的红覆盆子芽提取物的抗氧化活性。方法:采用分光光度法测定红覆盆子芽提取物中酚类化合物、儿茶素、类黄酮和羟基肉桂酸的含量，碱度法测定有机酸的含量;用电位法测定所得提取物的抗氧化活性。结果:红覆盆子芽总抗氧化能力为164.12 mmol-当量。/m干重时，红树莓芽提取物中酚类化合物的总含量为:没食子酸(GA) 24.40 mg /mL，儿茶素(EGCG) 21.36 mg /mL，黄酮类化合物(芦丁)0.77 mg /mL，羟基肉桂酸衍生物绿原酸(ChA) 2.56 mg /mL，有机酸(CA) 1.88 mg /mL。分析表明，红树莓芽提取物的抗氧化活性与总酚类化合物、儿茶素、类黄酮、羟基肉桂酸衍生物和有机酸含量呈正相关。结论:确定了红覆盆子总芽的抗氧化能力。研究结果可为开发具有抗氧化作用的红覆盆子芽提取物的最佳提取工艺提供依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Vitae PHARMACOLOGY & PHARMACY-

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal VITAE is the four-monthly official publication of the School of Pharmaceutical and Food Sciences, and its mission is the diffusion of the scientific and investigative knowledge in the various fields of pharmaceutical and food research, and their related industries. The Journal VITAE is an open-access journal that publishes original and unpublished manuscripts, which are selected by the Editorial Board and then peer-reviewed. The editorial pages express the opinion of the Faculty regarding the various topics of interest. The judgments, opinions, and points of view expressed in the published articles are the responsibility of their authors.