Isolation and Molecular Identification of Trichophyton rubrum var. raubitschekii from the Infant Groin

Abstract

Five clinical isolates of Trichophyton rubrum isolated from patients withdermatophytosis in the Dermatology unit of the General Hospital in Kalar distric/Sulaimaniprovince/North region of Iraq. The isolates were identified according to the conventionallaboratory methods and molecular methods, one of these strains was isolated from the groinof an infant with one year old and identifies as Trichophyton rubrum var. raubitschekii. Thisvariety characterized by the production of cottony to a velvety colony. The front of the colonyappeared brown color with brownish pigmentation at the reverse. Slide examination revealednumerous cylindrical shaped microconidia and few longer macroconidia. Molecularidentification was conducted according to the conventional PCR by using set primers ITS1and ITS4 and resulted in PCR product about 690bp. PCR-RFLP by using BstN1 digestionenzyme revealed three pattern bands 380,180,100bp. While sequencing of the ITS regionrevealed high similarity with that of a number of uncultured endocytic fungus clone.