In vivo changes in free choline level induced by autonomic agonists in mouse organs, including three major salivary glands

Takaki Kawaguchi , Shigeo Murai , Hiroko Saito
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引用次数: 4

Abstract

Whether free choline levels are changeable in vivo in response to different types of autonomic agonists was examined in several mouse organs. Upon one subcutaneous injection of isoproterenol, phenylephrine and pilocarpine, choline levels in whole organ decreased, increased and decreased, respectively, in various organs within 30 min and returned to initial levels in a day. In the three major salivary glands, a delayed choline elevation also appeared on day 2 after one isoproterenol injection and subsided by day 6. Only in the three salivary glands more choline was accumulated after 10 once-a-day injections of isoproterenol than after one isoproterenol injection. Neither phenylephrine nor pilocarpine induced comparable choline accumulation in any organs examined. Isoproterenol injection repeated at a 2-day interval augmented the subsequent, delayed choline elevation. Examination with dobutamine and the adenylyl cyclase activator 6-(3-dimethylaminopropionyl)forskolin suggested that isoproterenol-induced immediate choline lowering was downstream of cAMP synthesis and linked to cAMP more tightly than the choline accumulation, though both choline changes occurred via β1-adrenergic receptors. Choline levels in the salivary glands also changed depending on the form of diet given and particularly in the parotid gland in parallel with gland weights. These results provide the first evidence for the autonomic control of intracellular choline levels; intracellular choline levels might be an integral part of the autonomic signalling pathway.

自主神经激动剂诱导小鼠器官(包括三个主要唾液腺)游离胆碱水平的体内变化
在不同类型的自主神经激动剂作用下,体内游离胆碱水平是否会发生变化?一次皮下注射异丙肾上腺素、苯肾上腺素和匹罗卡品后,各器官胆碱水平在30分钟内分别下降、升高和降低,并在一天内恢复到初始水平。在三个主要唾液腺中,一次异丙肾上腺素注射后第2天也出现迟发性胆碱升高,第6天消退。只有在三个唾液腺中,每天一次注射异丙肾上腺素10次后积累的胆碱多于一次注射异丙肾上腺素。在检查的任何器官中,苯肾上腺素和匹罗卡品都没有引起类似的胆碱积累。每隔2天重复注射异丙肾上腺素可增强随后迟发的胆碱升高。多巴酚丁胺和腺苷酸环化酶激活剂6-(3-二甲氨基丙酰)福斯科林的检测表明,异丙肾上腺素诱导的胆碱立即降低位于cAMP合成的下游,与胆碱积累的联系更紧密,尽管这两种胆碱的变化都是通过β1-肾上腺素能受体发生的。唾液腺中的胆碱水平也会随着饮食的形式而变化,尤其是腮腺中的胆碱水平与腺体体重平行变化。这些结果为细胞内胆碱水平的自主控制提供了第一个证据;细胞内胆碱水平可能是自主神经信号通路的一个组成部分。
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