VIABILITY OF DUAL-SPECIES BIOFILM OF STREPTOCOCCUS MUTANS AND LACTOBACILLUS ACIDOPHILUS AFTER APPLICATION OF MAULI BANANA STEM GEL

Dentino Jurnal Kedokteran Gigi Pub Date : 2022-04-04 DOI:10.20527/dentino.v7i1.13104

A. N. Carabelly, Dhya Aurellia Salsabila Karno, Isyana Erlita, Aninditya Pimas Trianuanty

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Abstract

Introduction: Dual-species biofilm of Streptococcus mutans and Lactobacillus acidophilus in dental caries has a higher viability or ability to survive in comparison to mono-species biofilm, consequently it is more resistant to antibiofilm. Mauli banana stem gel (MBSG) contains isoleucine, cinnamic acid, and another bioactive constituents that may work as antibiofilm, hence it is able to decrease the viability of dual-species biofilm of S. mutans and L. acidophilus. This research aims to analyze the effect of MBSG on viability of dual-species biofilm of S. mutans and L. acidophilus. Material and methods: This research is a true experimental research with post test only with control group design which divides dual-species biofilm of S. mutans and L. acidophilus into 12 groups with 4 replications. Group 1 are given 0.2% chlorhexidine gel (positive control), group 2 are not given any treatment (negative control), group 3 are the media control, and group 4-12 are given MBSG concentration of 6.25%, 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 98%. Each group is incubated for 72 hours, afterward is given MTT reagent and read using a microplate reader. Results: The result of Kruskal-Wallis (p=0,00) and Mann-Whitney test showed significant difference between all treatment groups and control groups. Conclusion: MBSG concentration of 6.25%, 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 98% are able to decrease the viability of dual-spesies biofilm of S. mutans and L. acidophilus.

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毛利香蕉茎凝胶对变形链球菌和嗜酸乳杆菌双种生物膜活性的影响

简介:龋齿中变形链球菌和嗜酸乳杆菌的双种生物膜比单种生物膜具有更高的生存能力或生存能力，因此对抗生素膜具有更强的耐药性。毛利香蕉茎凝胶(Mauli banana stem gel, MBSG)含有异亮氨酸、肉桂酸等具有抗生物膜作用的活性成分，能够降低变形链球菌和嗜酸乳杆菌双种生物膜的活性。本研究旨在分析MBSG对变形链球菌和嗜酸乳杆菌双种生物膜活力的影响。材料与方法:本研究是一项真正的实验研究，仅进行后测，采用对照组设计，将变形链球菌和嗜酸乳杆菌双种生物膜分为12组，每组4个重复。1组给予0.2%氯己定凝胶(阳性对照)，2组不给予任何处理(阴性对照)，3组为培养基对照，4-12组分别给予浓度为6.25%、12.5%、25%、37.5%、50%、62.5%、75%、87.5%、98%的MBSG。每组孵育72小时，然后给予MTT试剂，用微孔板读取仪读取。结果:各治疗组与对照组的Kruskal-Wallis检验(p=0 000)和Mann-Whitney检验结果均有显著性差异。结论:浓度为6.25%、12.5%、25%、37.5%、50%、62.5%、75%、87.5%和98%的MBSG均能降低变形链球菌和嗜酸乳杆菌双菌种生物膜的活力。

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Dentino Jurnal Kedokteran Gigi

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24 weeks