Study on the effect of maternal administration of oxaliplatin on offspring testes using unbiased design-based stereology

J. Sadeghinezhad, Moslem Dahmardeh, Z. Tootian, Hadis Bojarzadeh, F. Yarmahmoudi
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引用次数: 3

Abstract

Oxaliplatin (Ox) is widely used for the treatment of various tumors. Since Ox prevents DNA replication and transcription, it may affect organs with rapid cell divisions such as the testes. Although its use during pregnancy has been reported, no information regarding its effects on the testes of the offspring is not available yet. Thirty-two mice were randomly divided into four groups. The control group (1) was administered intraperitoneally 0.2 ml of saline three times a week for the 21 days of pre-pregnancy, pregnancy and lactation. Experimental groups 2, 3 and 4 received 3 mg/kg of oxaliplatin three times a week for 21 days during pre-pregnancy, pregnancy and lactation, respectively. The left testis was removed from male offspring 30 and 60 days after birth. The volume of the testes, seminiferous tubules and interstitial tissue, the surface area and height of the seminiferous epithelium, as well as the length and diameter of seminiferous tubules, were analyzed by means of stereology. Results showed a decrease in the evaluated parameters in experimental groups, in comparison with the control group. Due to the ameliorating effect of Ox on offspring testes, cautiousness is needed during maternal administration in order to preserve the fertility of male offspring.
利用无偏设计体视学研究母体给予奥沙利铂对子代睾丸的影响
奥沙利铂广泛用于各种肿瘤的治疗。由于Ox会阻止DNA的复制和转录,因此可能会影响睾丸等细胞分裂迅速的器官。虽然在怀孕期间使用它的报道,但目前还没有关于它对后代睾丸影响的信息。32只小鼠随机分为四组。对照组(1)在孕前、妊娠期和哺乳期21 d,每周3次腹腔注射生理盐水0.2 ml。实验2、3、4组在妊娠前期、妊娠期和哺乳期分别给予奥沙利铂3 mg/kg,每周3次,连用21 d。雄性后代在出生后30天和60天切除左睾丸。用体视学方法分析睾丸、精管和间质组织的体积,精管上皮的表面积和高度,精管的长度和直径。结果显示,与对照组相比,实验组的评估参数有所下降。由于牛对子代睾丸有改善作用,为了保持雄性后代的生育能力,在母体给药时需谨慎。
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