An analytical tooth model based on SPR chips coated with hydroxyapatite used for investigation of the acquired dental pellicle

Physica status solidi (A): Applied research Pub Date : 2023-09-05 DOI:10.1002/pssa.202300146

D. Vornicescu, V. Penta, Michael Keusgen

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Abstract

One of the main targets in modern dentistry is the prevention of caries. Not only daily nutrition plays an important role, but also the so called “pellicle” on the outer surfaced of teeth, which is mainly formed by saliva proteins. Moreover, numerous bacteria are part of this pellicle, which might cause caries by acidic metabolites. In the here presented study, a method for electrophoretic deposition (EPD) of hydroxyapatite (HAP) nanoparticles on gold surfaces, suitable for Surface Plasmon Resonance (SPR) measurements has been developed. An “artificial tooth” has been created and loaded by natural saliva in different concentrations (to form a natural pellicle), while the influence of an environment with different pH values has been studied. It could be demonstrated that even slight acid solutions damage the pellicle significantly within seconds, exposing the HAP to acidic degradation, which would lead to caries in the human oral cavities. This model allows to study pellicle formation as well as degradation in real time. As a practical example, the influence of beverages on the pellicle could be demonstrated.This article is protected by copyright. All rights reserved.

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基于羟基磷灰石包覆SPR芯片的牙齿分析模型用于研究获得性牙膜

现代牙科的主要目标之一是预防龋齿。不仅日常营养起着重要作用，牙齿外表面的所谓“外膜”也起着重要作用，它主要是由唾液蛋白质形成的。此外，许多细菌是这一膜的一部分，它们可能通过酸性代谢物引起龋齿。在本研究中，开发了一种适合表面等离子体共振(SPR)测量的羟基磷灰石(HAP)纳米颗粒在金表面的电泳沉积(EPD)方法。用不同浓度的天然唾液制造和装载一颗“人造牙齿”(形成天然的牙膜)，同时研究了不同pH值环境的影响。可以证明，即使是轻微的酸性溶液，也会在几秒钟内显著破坏口腔膜，使HAP暴露于酸性降解中，从而导致人类口腔龋齿。该模型可以实时研究膜的形成和降解。作为一个实际的例子，可以证明饮料对薄膜的影响。这篇文章受版权保护。版权所有。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Physica status solidi (A): Applied research

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