Structural genomics on metalloproteins

Abstract

Metalloproteins constitute a significant share of the proteome. Their structure determination and protein-protein interaction studies allow investigation of the role of metal cofactors and interpretation of the biophysical features and the biological function. The approach is that of genome browsing, expression of a few representative samples and partner proteins, structure determination (mainly by NMR, through quick protocols), and modeling of the other homologous proteins. The analysis of structures which in turn connect genes to protein function is presented for some proteins involved in copper homeostasis and for some classes of cytochromes c and ferredoxins.