CONSERVATION OF CHOLERA VIBRIOS IN COMPLEX MICROCOSM CONTAINING GREEN MICROALGAE

S. Titova, E. Menshikova, Sergei Olegovich Vodop'yanov, Oksana Vasilyevna Borodina, Artem A. Gerasimenko, I. P. Oleynikov, Nadechda Alexandrovna Selyanskaya
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Abstract

BACKGROUND. In the summer-autumn period, the peak of reproduction and flowering of phytoplankton occurs, as a result of which, during this period, a deterioration in the quality of river water is noted. At the same time, cases of gastrointestinal diseases in humans are recorded, and epidemic complications of cholera may occur in endemic areas. The formation of biofilm forms by V. cholerae on the surface of chitinous hydrobionts and plastic components can lead to the spread of V. cholerae and possibly explains the autochthonous mechanism of their existence in water bodies. AIM to determine the duration of vibrio cholerae preservation on biotic (chitin) and abiotic (plastic) substrates in the presence of green unicellular algae with a change in cultivation temperature, under experimental conditions. MATERIALS AND METHODS. to achieve this goal, bacteriological and molecular genetic methods were used. RESULTS. It was shown that the preservation of toxigenic and non-toxigenic strains of V. cholerae O1 El Tor and V. cholerae O139 (ctxAB+ tcpA+csh1- and ctxA tcpAcsh1+) for six months, including three months at a low temperature simulating autumn winter period, as part of biofilms in microcosms, where one of the components is green microalgae. We noted an excess of the concentration of V. cholerae O1 and O139 serogroups by two orders of magnitude in samples, where one of the components is chitin and the reproduction of green microalgae in the presence of a chitin substrate, which is probably one of the stages of the food chain in the ecology of water bodies and, accordingly, can be a reservoir for biofilm forms of cholera vibrios. Preservation of V. cholerae O1 El Tor non-toxigenic strain in a viable state in biofilm samples on plastic at a decrease in temperature to 8 2C for three months is possibly due to the presence of a cold shock gene in its genome. CONCLUSION. Without a substrate for adhesion, colonization and biofilm formation, vibrios are not capable of long-term persistence at low temperatures
含绿色微藻的复杂微环境中霍乱弧菌的保存
背景。夏秋季节是浮游植物繁殖和开花的高峰期,因此,在此期间,河流水质出现了恶化。与此同时,人类胃肠道疾病的病例也有记录,霍乱的流行并发症可能在流行地区发生。霍乱弧菌在几丁质水生生物和塑料组分表面形成生物膜可导致霍乱弧菌的传播,这可能解释了霍乱弧菌在水体中存在的原生机制。目的在实验条件下,研究绿色单细胞藻类存在时,霍乱弧菌在生物(几丁质)和非生物(塑料)基质上随培养温度变化的保存时间。材料和方法。为了实现这一目标,使用了细菌学和分子遗传学方法。结果。结果表明,产毒株和非产毒株霍乱弧菌O1 El Tor和霍乱弧菌O139 (ctxAB+ tcpA+csh1-和ctxA tcpAcsh1+)作为生物膜的一部分在模拟秋冬期的低温环境中保存6个月,其中3个月的生物膜成分之一是绿色微藻。我们注意到样品中霍乱弧菌O1和O139血清群的浓度超过两个数量级,其中一种成分是几丁质,绿色微藻在几丁质底物存在的情况下繁殖,这可能是水体生态食物链的一个阶段,因此可能是霍乱弧菌生物膜形式的储存库。霍乱弧菌O1 El - Tor非产毒菌株在塑料生物膜样品中以活菌状态保存3个月,温度降至8℃可能是由于其基因组中存在冷休克基因。结论。没有附着、定植和生物膜形成的底物,弧菌不能在低温下长期生存
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