Effects of lipopolysaccharide on interleukin-6 production in perfused human placental cotyledons†

The Journal of maternal-fetal medicine Pub Date : 2001-02-15 DOI:10.1002/1520-6661(200011/12)9:6<351::AID-MFM1006>3.0.CO;2-5

Richard K. Wagner, Roger M. Hinson, Christina C. Apodaca, Nathan Hoeldtke, Trey Buchanan, Roderick F. Hume Jr., Byron C. Calhoun

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引用次数: 2

Abstract

Objective

To determine if lipopolysaccharide (LPS) alters production of interleukin-6 (IL-6) or vascular tone in perfused placental cotyledons.

Methods

Control and study cotyledons from nine placentas were perfused for 3 h. Study cotyledons received LPS in concentrations of 0.01 mcg/ml (n = 3), 0.1 mcg/ml (n = 3), or 1.0 mcg/ml (n = 3). Effluents were collected at 30, 60, 120, and 180 min following infusion with LPS. IL-6 concentrations were measured by enzyme-linked immunosorbant assay. Perfusion pressures were recorded at 10-min intervals. Data were analyzed using ANOVA for repeated measures.

Results

IL-6 production significantly increased over time in both the study and control cotyledons (P = 0.002). LPS treatment did not affect IL-6 production (P = 0.85) and there were no observable dose effects (P = 0.13). Perfusion pressures did not differ (P = 0.16).

Conclusions

The isolated perfused placental cotyledon produces IL-6 and concentrations increase over time. LPS does not alter production of IL-6 or fetoplacental vascular tone. J. Matern.-Fetal Med. 2000;9:351–355. © 2000 Wiley-Liss, Inc.

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脂多糖对灌注人胎盘子叶白细胞介素-6生成的影响

目的探讨脂多糖(LPS)是否会改变灌注胎盘子叶中白细胞介素-6 (IL-6)的产生或血管张力。方法将9个胎盘的对照子叶和研究子叶灌注3 h，研究子叶分别接受浓度为0.01 mcg/ml (n = 3)、0.1 mcg/ml (n = 3)和1.0 mcg/ml (n = 3)的LPS，分别于LPS灌注后30、60、120和180 min收集流出液。采用酶联免疫吸附法测定IL-6浓度。每隔10分钟记录灌注压力。重复测量数据采用方差分析。结果随着时间的推移，IL-6在研究子叶和对照子叶中的产量均显著增加(P = 0.002)。LPS处理不影响IL-6的产生(P = 0.85)，且无明显剂量效应(P = 0.13)。灌注压力无差异(P = 0.16)。结论离体灌注胎盘子叶可产生IL-6，且IL-6浓度随时间升高。LPS不改变IL-6的产生或胎儿胎盘血管张力。j . Matern。-胎儿医学，2000;9:31 - 355。©2000 Wiley-Liss, Inc。

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来源期刊

The Journal of maternal-fetal medicine

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