Production and Purification of Laccase Enzyme by Klebsiella pneumoniae K7

chemistry and materials research Pub Date : 2020-05-01 DOI:10.7176/cmr/10-5-03

Doaa Khalid Mezaal, E. F. Al-Jumaili, Ahmed Majeed Al-Shammari

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Abstract

Sixty-four isolate were klebsiella pneumoniae. Fourteen bacteria isolates “ Kelbsiella species” were taken from soil and water hospital in the period between October to December 2018, those isolated were cultured on a blood agar to test their ability to hydrolytic due to formation the inhibition zone . Twenty one isolates of K. pneumoniae were selected to be cultured in mineral salt agar for testing their efficiency to produce laccase enzyme .The efficient isolate was diagnosed depending on phenotypic, microscopic and biochemical tests to be Klebsiella pneumoniae K7. Laccases (benzenediol: oxygen oxidoreductases; EC: 1.10.3.2) are subfamily of multicopper oxidases (MCOs) from Klebsiella pneumoniae K7 has been partially characterized by many researchers. In this paper, we purified laccase to homogeneity from Klebsiella pneumoniae K7 with about 10.82 ; 5.12 purification fold and a 34.14; 21.46% recovery by ion-exchange and gel-filtration chromatographic respectively. The molecular weight of the Laccase as determined by gel filtration chromatography using Sephacryl S-200 gel was 120 KDalton. Keywords: Laccases , Klebsiella pneumoniae, molecular weight, purification. DOI: 10.7176/CMR/10-5-03 Publication date: May 31 st 2020

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肺炎克雷伯菌K7漆酶的制备与纯化

64株分离物为肺炎克雷伯菌。2018年10月至12月，从土壤和水医院中分离出14株“克尔伯氏菌种”，在血琼脂上培养，检测其因形成抑制带而产生的水解能力。选择21株肺炎克雷伯菌在无机盐琼脂培养基中培养，测定其产漆酶的效率，经表型、镜检和生化检查，鉴定为肺炎克雷伯菌K7。苯二醇漆酶:氧氧化还原酶;EC: 1.10.3.2)是来自肺炎克雷伯菌K7的多铜氧化酶(MCOs)亚家族，许多研究者已经对其进行了部分表征。本文从肺炎克雷伯菌K7中纯化漆酶，纯度约为10.82;5.12纯化折叠和34.14;离子交换色谱和凝胶过滤色谱回收率分别为21.46%。用Sephacryl S-200凝胶过滤层析法测定漆酶的分子量为120 KDalton。关键词:漆酶，肺炎克雷伯菌，分子量，纯化出版日期:2020年5月31日

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chemistry and materials research

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