The utility of qNMR to improve accuracy and precision of LC-MS bioanalysis

Abstract

The constantly evolving complexities of the drug discovery and development process necessitate continued advancements of supporting LC-MS bioanalytical assays by improving critical analytical figures of merit such as specificity and sensitivity along with accuracy and precision (Figure 1). The principal objective of quantitative assays is to correctly and consistently/repeatedly measure the real values thus affording quality data with high accuracy and high precision. In some cases, when only a change of the levels of endogenous compounds from baseline is monitored, an assay with low accuracy and high precision may be sufficient [1,2]. Occasionally, assays with high accuracy and low precision or even assays with low accuracy and low precision might suitable for fit-for-purpose analysis. In LC-MS bioanalysis, dry reference standards are used to prepare stock solutions followed by serial dilution to provide calibration solutions and curves against which the samples and analytes are then quantified. The analytical errors interrelated to the mass and volume measurements needed for the preparation of these solutions JOURNAL OF APPLIED BIOANALYSIS, January 2018, p. 26-31. http://dx.doi.org/10.17145/jab.18.005 (ISSN 2405-710X) Vol. 4, No. 1