Expression level and clinical significance of serum exosomal miR-744-5p in non-small cell lung cancer

Q4 Health Professions

中华检验医学杂志 Pub Date : 2020-02-11 DOI:10.3760/CMA.J.ISSN.1009-9158.2020.02.008

Shao-Ping Li, Xin Zhang, Han-Chao Zhang

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Abstract

Objective To analysis the expression level and clinical value of serum exosomal miR-744-5p in non-small cell lung cancer (NSCLC). Methods Retrospective study. A total of 183 subjects, including 92 NSCLC patients and 91 healthy controls, were recruited from Nanfang Hospital Affiliated to Southern Medical University from November 2016 to February 2018. Exosomes were extracted using ExoQuick and the total RNA was extracted from the exosomes.The expression level of serum exosomal miR-744-5p was assessed by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The diagnostic efficiency of exosomal miR-744-5p was evaluated by receiver operating characteristic (ROC) curve. Results The qRT-PCR results revealed that the expression level of serum exosomal miR-744-5p in patients with NSCLC (0.012 2±0.019 7) was significantly lower than that in the healthy control (0.093 6±0.081 9), and the difference was statistically significant (U=553.5, P<0.001). Furthermore, the level of miR-744-5p in patients with early stage (stage Ⅰ and Ⅱ) lung cancer(0.011 7±0.013 9) was significantly different, when compared to healthy controls (U=72.5, P<0.001). The exosomal level of miR-744-5p in samples (0.010 6±0.020 3) was lower than that in squamous cell carcinoma serum (0.017 3±0.017 8) , and the difference was statistically significant (U=394.5, P<0.001). The ROC curve revealed that miR-744-5p expression can be a significant biomarker to discriminate between normal and NSCLC cases, with an area under the ROC curve of 0.933 9 (95%CI=0.899 6-0.968 1, P<0.000 1). MiR-744-5p can effectively diagnose early stage NSCLC, with an area under the ROC curve of 0.943 1 (95%CI=0.883 7-1.000 0, P<0.000 1). Conclusion The serum exosomal miR-744-5p could serve as a non-invasive biomarker for the diagnosis of NSCLC, but further comparative research and expanded verification are needed. Key words: Carcinoma, non-small-cell lung; Exosomes; microRNAs; Biomarkers, tumor

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血清外泌体miR-744-5p在非小细胞肺癌中的表达水平及临床意义

目的分析血清外泌体miR-744-5p在非小细胞肺癌(NSCLC)中的表达水平及临床价值。方法回顾性研究。2016年11月至2018年2月，南方医科大学附属南方医院共招募了183名受试者，其中包括92名非小细胞肺癌患者和91名健康对照。使用ExoQuick提取外泌体并从外泌体中提取总RNA。采用实时荧光定量聚合酶链反应(qRT-PCR)检测血清外泌体miR-744-5p的表达水平。采用受试者工作特征(ROC)曲线评价外泌体miR-744-5p的诊断效能。结果qRT-PCR结果显示，NSCLC患者血清外泌体miR-744-5p表达水平(0.012 2±0.019 7)显著低于健康对照组(0.093 6±0.081 9)，差异有统计学意义(U=553.5, P<0.001)。此外，早期(Ⅰ和Ⅱ期)肺癌患者miR-744-5p水平(0.011 7±0.013 9)与健康对照组相比差异有统计学意义(U=72.5, P<0.001)。样本中miR-744-5p外泌体水平(0.010 6±0.020 3)低于鳞状细胞癌血清(0.017 3±0.017 8)，差异有统计学意义(U=394.5, P<0.001)。ROC曲线显示miR-744-5p的表达可作为区分正常和非小细胞肺癌的重要生物标志物，其ROC曲线下面积为0.933 9 (95%CI=0.899 6 ~ 0.968 1, P<0.000 1)， miR-744-5p可有效诊断早期非小细胞肺癌，其ROC曲线下面积为0.943 1 (95%CI=0.883 7 ~ 1.000 0, P<0.000 1)。结论血清外体miR-744-5p可作为诊断非小细胞肺癌的无创生物标志物。但还需要进一步的比较研究和进一步的验证。关键词:肺癌，非小细胞肺;液;小分子核糖核酸;肿瘤生物标志物,

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