Secondary Metabolites from Mimosa Pudica: Isolation, Purification and NMR Characterization

Abstract

Aim: isolation, purification and NMR characterization of secondary metabolites in Mimosa pudica. Study design: extraction, isolation and identification of the secondary metabolties from the petroleum ether and metabolites extracts from Mimosa pudica. Methodology: In the course of our study on Mimosa pudica, a compound was isolated and purified using different chromatographic techniques and the structure of the compound determined on the basis of their spectroscopic data (IR, HNMR, COSY, DEPT, MS). Place and Duration of Study: this work was carried out in the chemistry Department Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria within the month June 2016 to December 2016. Result: The entire plant parts of Mimosa was extracted using absolute ethanol, the TLC of the crude extract indicate the many compounds with Rf values 0.41,0.43,0.56,0.68,0.89 and 0.90. Purification: The results of several chromatographic techniques on the isolated gave rise to a triterpenoidglycoside with Rf value 0.55. The spectral analyses involving HNMR, NMR, DEPT, 135 COSY, HMBC and IR, MS were useful in proposing the structure of the compound MP11.