Polygalacturonase (PG) production by fungal strains using agro-industrial bioproduct in solid state fermentation

Chemical Engineering Research Bulletin Pub Date : 2011-08-08 DOI:10.3329/CERB.V15I1.6368

H. Abbasi, Seyed Roholah Mortazavipour, Mehrdad Setudeh

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引用次数: 16

Abstract

Polygalacturonase production by isolated fungal strains was carried out in solid state fermentation. Aspergillus niger ( A. niger ) and Penicillium sp EGC5 produced polygalacturonase (PG) on mixture of apple bagasse and wheat bran. The mixture of apple bagasse and wheat bran acted as a good nutrient source and substrate for the cultivation of the microorganisms and polygalacturonase produced in solid state fermentation. In this respect, it was possible to obtain polygalacturonase activity at an acceptable yield, in comparison with a typical defined medium described in the literature for polygalacturonase production. Higher titres of polygalacturonase were observed when medium was supplemented with carbon (pectin) and nitrogen ((NH 4 ) 2 SO 4 and peptone) sources. The maximum production of polygalacturonase was reached after 8 days cultivation. The temperature was 30°C and the relative humidity of the fermentation medium was 70%. Key words: Polygalacturonase; Aspergillus niger; Apple bagasse; wheat bran; Solid state fermentation DOI: http://dx.doi.org/10.3329/cerb.v15i1.6368 Chemical Engineering Research Bulletin 15 (2011) 1-5

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农工生物制品固态发酵真菌生产聚半乳糖醛酸酶(PG)的研究

分离的真菌菌株在固态发酵中生产聚半乳糖醛酸酶。黑曲霉(Aspergillus niger)和青霉(Penicillium sp . EGC5)对苹果甘蔗渣和麦麸的混合物产生聚半乳糖醛酸酶(PG)。苹果甘蔗渣与麦麸的混合物为微生物的培养和固态发酵产生的聚半乳糖醛酸酶提供了良好的营养源和底物。在这方面，与文献中描述的聚半乳糖醛酸酶生产的典型定义培养基相比，有可能以可接受的产量获得聚半乳糖醛酸酶活性。当培养基中添加碳(果胶)和氮((nh4) 2so4和蛋白胨)源时，聚半乳糖醛酸酶的滴度较高。培养8天后，聚半乳糖醛酸酶产量达到最大值。温度为30℃，发酵培养基相对湿度为70%。关键词:聚半乳糖醛酸酶;黑曲霉;苹果蔗渣;小麦麸皮;固体发酵DOI: http://dx.doi.org/10.3329/cerb.v15i1.6368化学工程研究通报15 (2011)1-5

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Chemical Engineering Research Bulletin

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