Role of β-Cryptoxanthin as an Antioxidant and Its Ability to Bind with Transferrin

Anushka Ghosh, U. Hazra, D. Dutta
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引用次数: 1

Abstract

The potential nutraceutical benefits of carotenoids, which are versatile bioactive compounds, have been of great interest recently for its applications as dietary supplements. Carotenoids are red-orange coloured pigments which absorb light in the wavelength region of 400-550 nm. They are produced by plants, bacteria, algae and fungi and are abundantly distributed in the nature. In this study, our focus is on beta-cryptoxanthin (β-CRX), a yellow colored pro-vitamin A xanthophyll which is extracted from a previously isolated bacterium Kocuria marina DAGII grown in Brain Heart Infusion and sub-cultured in low cost dairy waste like whey and incubated at 25°C and 150 rpm for 5 days. The extracted beta-cryptoxanthin showed good radical scavenging activity and played a role in inhibition of lipid oxidation. Transferrin is a glycoprotein which plays a significant role in the mobilisation of iron in the body. It has two receptors TfR1 and TfR2 amongst which TfR1 binds to the iron-loaded transferrin. In cases of secondary hemochromatosis, HFE protein competes with transferrin to bind to TfR1 which leads to iron built up which is detrimental to the human body. Beta-cryptoxanthin was found to bind to Transferrin with a binding energy of -8.2 kcal/mol.
β-隐黄质作为抗氧化剂的作用及其与转铁蛋白结合的能力
类胡萝卜素是一种多用途的生物活性化合物,其潜在的营养益处最近因其作为膳食补充剂的应用而引起了极大的兴趣。类胡萝卜素是红橙色的色素,吸收波长在400-550纳米之间的光。它们由植物、细菌、藻类和真菌产生,在自然界中分布丰富。在这项研究中,我们的重点是β-隐黄质(β-CRX),这是一种黄色的维生素a原叶黄素,从先前分离的细菌Kocuria marina DAGII中提取,该细菌在脑心脏灌注中生长,并在乳清等低成本乳制品废物中进行传代培养,并在25°C和150 rpm下孵育5天。提取的-隐黄质具有良好的自由基清除活性,并具有抑制脂质氧化的作用。转铁蛋白是一种糖蛋白,在体内铁的动员中起着重要作用。它有两个受体TfR1和TfR2,其中TfR1与载铁转铁蛋白结合。在继发性血色素沉着症的病例中,HFE蛋白与转铁蛋白竞争,与TfR1结合,导致铁积聚,对人体有害。β -隐黄质与转铁蛋白结合,结合能为-8.2 kcal/mol。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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