Assesment of molecular diversity of internal transcribed spacer region in some lines and landrace of Persian clover (Trifolium resupinatum L.)

Q3 Agricultural and Biological Sciences

Potravinarstvo Pub Date : 2018-10-16 DOI:10.5219/960

S. Ansari, M. Solouki, B. Fakheri, B. Fazeli-Nasab, N. Mahdinezhad

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引用次数: 7

Abstract

Clover which is an herbaceous, annual, and self-pollinated plant belongs to fabaceae family (legumes) and has become naturalized in Iran, Asia Minor and the Mediterranean eastern suburban countries. The aim of the present study is ITS molecular evaluation of the nuclear ribosomal genes of lines and landraces of Persian Clover. The sequences were aligned using ClustalW method and by MegAlign software and the dendrogram of different phylogenetic and matrix relationships between the sequences were drawn. The results showed little genetic diversity between the lines and the landrace. The conserved sequence of the analyzed gene in the Persian clover is 561 base. Totally, 740 loci (69 and 671 loci, respectively, with and without removal and addition), 9 Singletons, and 5 haplotypes were identified. The highest rate of transfer was observed in pyrimidine (%16.3). The numerical value of the ratio (dN/dS) was 0.86, and since it was less than 1, the pure selection on the studied gene happened. The lines and landraces were not separated based on their geographic locations. In general, the results indicated that the highest rate of the regional diversity belonged to the clover plants in Lorestan region. Moreover, ITS markers did not seem suitable enough for evaluating the intra- species genetic variation, but it was quite well- suited for inter-species or intergeneric evaluation. Normal 0 false false false EN-GB X-NONE AR-SA

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波斯三叶草(Trifolium resupinatum L.)部分品系和地方品种内部转录间隔区的分子多样性评价

三叶草是一种草本，一年生，自花授粉的植物，属于豆科(豆科)，已归化在伊朗，小亚细亚和地中海东部郊区国家。本研究的目的是对波斯三叶草系和地方品种核糖体基因进行ITS分子评价。利用ClustalW方法和MegAlign软件对序列进行比对，绘制不同系统发育的树状图和序列间的矩阵关系。结果表明，这些品系与地方品种之间的遗传多样性很小。该基因在波斯三叶草中的保守序列为561个碱基。共鉴定出740个位点(分别为69个和671个)，9个单型和5个单倍型。转移率最高的是嘧啶(%16.3)。该比值(dN/dS)的数值为0.86，由于该比值小于1，对所研究的基因进行了纯选择。界线和地方种族并不是根据他们的地理位置分开的。总体而言，洛丽斯坦地区三叶草植物的区域多样性率最高。此外，ITS标记对种内遗传变异的评价似乎不太合适，但对种间或属间遗传变异的评价却很适合。正常0 false false false EN-GB X-NONE AR-SA

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来源期刊

Potravinarstvo Agricultural and Biological Sciences-Food Science

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Potravinarstvo Slovak Journal of Food Sciences. Articles published in the journal are peer reviewed and freely available online. Journal covers areas including: food hygiene, food safety and quality, food microbiology, food laws and regulations, ingredients and ingredient functionality, nutraceuticals, product formulation, sensory science and sensory statistical analysis, process control and its contribution to food processing operations, food chemistry, food toxicology, food engineering, food technology and biotechnology, nourishment, public health, primary production of food, food adulteration, food economics and marketing, environmental effect on food and food machinery.