ANTIMICROBIAL AND ANTIRADICAL PROPERTIES OF HAMMADA SCOPARIA (POMEL) ILJIN

African Journal of Traditional, Complementary and Alternative Medicines Pub Date : 2020-01-09 DOI:10.21010/ajtcam.v16n2.1

A. Drioiche, N. Benhlima, S. Kharchouf, F. E. Makhoukhi, S. Mehanned, Imad Adadi, Hicham Aaziz, Ferdinand Kouoh Elombo, B. Gressier, B. Eto, T. Zair

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引用次数: 4

Abstract

Background: Hammada scoparia (Pomel) Iljin (HS), is commonly used by traditional healers in Morocco against microbial and fungal infections. We studied antimicrobial, antifungal and antiradical effects of organic extracts in vitro in order to confirm traditional utilization after phytochemical screening. Materials and methods: Aerial parts of HS have been extracted by hydro-distillation using Clevenger-type apparatus, and the chemical composition was realized by Gas Chromatography coupled with Mass Spectroscopy (GC/MS). The antioxidant activity has been evaluated using DPPH test, while the antimicrobial tests of HS extract were conducted on twenty-eight bacterial strains and antifungal on twelve fungal strains. Results: Chemical characterization of HS essential oils (EO) confirmed the presence of carvacrol (82,28%), p-cymene (2,52%), γ-terpinene (2,18%) and Z-caryophyllene (2,04%). Antimicrobial tests of HS extract showed a moderate antibacterial activity without antifungal effect. In addition, HS exhibited a very powerful antiradical activity with IC50 = 1,2 mg/ml compared to that of ascorbic acid (IC50 = 0,5 mg/mL) and butylated hydroxyanisole (0,8 mg/mL). Conclusion: To our knowledge, this is the first demonstration that HS directly inhibits the growth of microorganisms in vitro, and further validates its traditional use as an antiseptic by traditional Moroccan healers.

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茯苓的抗菌和抗自由基作用

背景:马莨菪(Pomel) Iljin (HS)是摩洛哥传统治疗师常用的治疗微生物和真菌感染的药物。通过对有机提取物的体外抗菌、抗真菌和抗自由基作用的研究，确定其传统的利用途径。材料与方法:采用Clevenger-type装置，采用水蒸气蒸馏法提取HS的空中部分，采用气相色谱-质谱联用(GC/MS)技术进行化学成分分析。采用DPPH试验对其抗氧化活性进行了评价，并对28株细菌进行了抑菌试验，对12株真菌进行了抑菌试验。结果:香芹精油(EO)的化学成分鉴定证实了香芹醇(82,28%)、对伞花烃(2,52%)、γ-萜烯(2,18%)和z -石竹烯(2,04%)的存在。抗微生物试验表明，HS提取物具有中等抑菌活性，无抗真菌作用。与抗坏血酸(IC50 = 0.5 mg/ml)和丁基羟基茴香醚(IC50 = 0.8 mg/ml)相比，HS具有较强的抗自由基活性(IC50 = 1.2 mg/ml)。结论:据我们所知，这是第一次在体外证明HS直接抑制微生物的生长，并进一步证实了其作为摩洛哥传统治疗师的防腐剂的传统用途。

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来源期刊

African Journal of Traditional, Complementary and Alternative Medicines 医学-全科医学与补充医学

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The “African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM)” is a peer-reviewed, multidisciplinary, international, scientific Open Access Journal that provides publication of articles on phytomedicines, ethnomedicines and veterinary ethnomedicines. The journal is published by a Non-Governmental Organization (NGO) known as “African Traditional Herbal Medicine Supporters Initiative (ATHMSI)”. The Journal welcomes submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published approximately two-to-three months after acceptance