Prevalence of blaTEM and blaVEB Genes in Multidrug-Resistant Acinetobacter baumannii Strains Isolated from Burn Wound Infection in Isfahan, Iran

Q4 Medicine

Infection Epidemiology and Microbiology Pub Date : 2020-06-01 DOI:10.29252/iem.6.2.109

Elham Haghighifar, R. Dolatabadi, A. Rezaei

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Determination of antibiotic resistance pattern and prevalence of OXA-type carbapenemases among Acinetobacter baumannii clinical isolates from inpatients in Isfahan, central ... [7] Rezaei A, Fazeli H, Halaji M, Moghadampour M, Faghri J. Prevalence of ... [8] Heidari H, Halaji M, Taji A, Kazemian H, Abadi MSS, Sisakht MT, et al. Molecular analysis of drug-resistant ... [9] García-Patiño MG, García-Contreras R, Licona-Limón P. The immune response against Acinetobacter baumannii, an ... [10] Kittinger C, Kirschner A, Lipp M, Baumert R, Mascher F, Farnleitner AH, et al. Antibiotic resistance of Acinetobacter spp. isolates from the river ... [11] JafariSales A, Bagherizadeh Y, Khalifehpour M, Abdoli-senejan M, Helali-Pargali R. Antibiotic resistance pattern and blaTEM gene expression in Acinetobacter baumannii isolated from clinical specimens ... [12] Ghaima KK, Abdulhassan AA, Mahdi ZH. Molecular study of ... [13] Torkar KG, Bedenić B. Antimicrobial susceptibility and characterization of metalloβ-lactamases, extended ... [14] Fazeli H, Taraghian A, Poursina F, Kamali R. Molecular ... [15] Sohail M, Rashid A, Aslam B, Waseem M, Shahid M, Akram M, et al. Antimicrobial ... [16] Rezai MS, Rafiei A, Ahangarkani F, Bagheri-Nesami M, Nikkhah A, Shafahi K, et al. Emergence ... [17] Burcu B, Acik L, Sultan L. Phenotypic and molecular ... [18] Vahidi Emami H, Khalilian M, Yadollahi Movahhed N. Antibiotic ... [19] Moosavian M, Sirous M, Shams N. Phenotypic and ... [20] Bali EB, Accedil L, Sultan N. Phenotypic ... [21] Salih TS, Shafeek RR. In silico detection ... [22] Abdar MH, Taheri-Kalani M, Taheri K, Emadi B, Hasanzadeh A, Sedighi A, et al. Prevalence of ... [23] Ahmadikiya F, Mosadegh A, Moradi M, Hossieni-Nave H. Antimicrobial ... [24] Azizi O, Shahcheraghi F. The frequency of blaPER, blaVEB, blaCTX-M, tetA and tetB genes among ... [25] Shahcheraghi F, Akbari Shahmirzadi N, Abbasalipour Bashash M, Jabbar H, Amirmozafari N. Detection ... [26] Safari M, Nejad ASM, Bahador A, Jafari R, Alikhani MY. Prevalence of ... Aims: Acinetobacter baumannii is an opportunistic pathogen that is resistant to many antibiotics including beta-lactams. Production of β-lactamases is the main mechanism of β-lactam resistance in A. baumannii strains. The aim of this study was to determine the frequency of blaTEM and blaVEB genes in clinical isolates of A. baumannii and the relationship between the antibiotic resistance and the presence of ESBL genes in strains isolated from burn wound infection in Isfahan. Materials & Methods: In this study, 123 MDR A. baumannii strains were isolated from burn wound infection. After antibiotic resistance evaluation using the Kirby-Bauer disc-diffusion method, all the isolates were evaluated with polymerase chain reaction (PCR) technique to detect ESBL genes, followed by statistical analysis by the end. Findings: Out of 123 A. baumannii isolates, 77 (62.60%) strains were ESBL positive according to the PCR results. The frequency of blaTEM and blaVEB genes was 52 (42.3%) and 67 (54.5%), respectively. There was a significant relationship between the antibiotic resistance and the presence of ESBL genes (blaTEM and blaVEB) in A. baumannii strains. Conclusion: The high prevalence of blaTEM and blaVEB genes in A. baumannii strains found in this study is the major concern about burn wound infections in Isfahan and Iran because of the complexity in treating infections caused by these strains. 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引用次数: 0

Abstract

Article History Received: March 15 ,2020 Accepted: May 15 ,2020 Published: June 10 ,2020 [1] Hashim IA, Wdaah QH, Atya AA. Potential effect of ... [2] Peck MD. Epidemiology of burns throughout the world. Part I: Distribution and ... [3] Capoor MR., Sarabahi S, Tiwari VK, Narayanan RP. Fungal ... [4] Zubair M, Malik A, Ahmad J, Rizvi M, Farooqui KJ, Rizvi MW. A study of ... [5] Asadollahi P, Akbari M, Soroush S, Taherikalani M, Asadollahi K, Sayehmiri K, et al. Antimicrobial resistance patterns and their encoding genes ... [6] Rezaei A, Fazeli H, Moghadampour M, Halaji M, Faghri J. Determination of antibiotic resistance pattern and prevalence of OXA-type carbapenemases among Acinetobacter baumannii clinical isolates from inpatients in Isfahan, central ... [7] Rezaei A, Fazeli H, Halaji M, Moghadampour M, Faghri J. Prevalence of ... [8] Heidari H, Halaji M, Taji A, Kazemian H, Abadi MSS, Sisakht MT, et al. Molecular analysis of drug-resistant ... [9] García-Patiño MG, García-Contreras R, Licona-Limón P. The immune response against Acinetobacter baumannii, an ... [10] Kittinger C, Kirschner A, Lipp M, Baumert R, Mascher F, Farnleitner AH, et al. Antibiotic resistance of Acinetobacter spp. isolates from the river ... [11] JafariSales A, Bagherizadeh Y, Khalifehpour M, Abdoli-senejan M, Helali-Pargali R. Antibiotic resistance pattern and blaTEM gene expression in Acinetobacter baumannii isolated from clinical specimens ... [12] Ghaima KK, Abdulhassan AA, Mahdi ZH. Molecular study of ... [13] Torkar KG, Bedenić B. Antimicrobial susceptibility and characterization of metalloβ-lactamases, extended ... [14] Fazeli H, Taraghian A, Poursina F, Kamali R. Molecular ... [15] Sohail M, Rashid A, Aslam B, Waseem M, Shahid M, Akram M, et al. Antimicrobial ... [16] Rezai MS, Rafiei A, Ahangarkani F, Bagheri-Nesami M, Nikkhah A, Shafahi K, et al. Emergence ... [17] Burcu B, Acik L, Sultan L. Phenotypic and molecular ... [18] Vahidi Emami H, Khalilian M, Yadollahi Movahhed N. Antibiotic ... [19] Moosavian M, Sirous M, Shams N. Phenotypic and ... [20] Bali EB, Accedil L, Sultan N. Phenotypic ... [21] Salih TS, Shafeek RR. In silico detection ... [22] Abdar MH, Taheri-Kalani M, Taheri K, Emadi B, Hasanzadeh A, Sedighi A, et al. Prevalence of ... [23] Ahmadikiya F, Mosadegh A, Moradi M, Hossieni-Nave H. Antimicrobial ... [24] Azizi O, Shahcheraghi F. The frequency of blaPER, blaVEB, blaCTX-M, tetA and tetB genes among ... [25] Shahcheraghi F, Akbari Shahmirzadi N, Abbasalipour Bashash M, Jabbar H, Amirmozafari N. Detection ... [26] Safari M, Nejad ASM, Bahador A, Jafari R, Alikhani MY. Prevalence of ... Aims: Acinetobacter baumannii is an opportunistic pathogen that is resistant to many antibiotics including beta-lactams. Production of β-lactamases is the main mechanism of β-lactam resistance in A. baumannii strains. The aim of this study was to determine the frequency of blaTEM and blaVEB genes in clinical isolates of A. baumannii and the relationship between the antibiotic resistance and the presence of ESBL genes in strains isolated from burn wound infection in Isfahan. Materials & Methods: In this study, 123 MDR A. baumannii strains were isolated from burn wound infection. After antibiotic resistance evaluation using the Kirby-Bauer disc-diffusion method, all the isolates were evaluated with polymerase chain reaction (PCR) technique to detect ESBL genes, followed by statistical analysis by the end. Findings: Out of 123 A. baumannii isolates, 77 (62.60%) strains were ESBL positive according to the PCR results. The frequency of blaTEM and blaVEB genes was 52 (42.3%) and 67 (54.5%), respectively. There was a significant relationship between the antibiotic resistance and the presence of ESBL genes (blaTEM and blaVEB) in A. baumannii strains. Conclusion: The high prevalence of blaTEM and blaVEB genes in A. baumannii strains found in this study is the major concern about burn wound infections in Isfahan and Iran because of the complexity in treating infections caused by these strains. This study results highlighted the need for infection control measures to prevent the spread of resistant isolates and ESBL genes, especially in burn hospitals.

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伊朗伊斯法罕烧伤感染多药耐药鲍曼不动杆菌株中blaTEM和blaVEB基因的流行

收稿日期:2020年3月15日收稿日期:2020年5月15日发布日期:2020年6月10日[1]哈希姆·IA, Wdaah QH, Atya AA。的潜在影响……[2]李建军，李建军。烧伤流行病学研究进展。第一部分:分布和……[3]刘建军，刘建军，李建军。真菌……[4]李建军，李建军，李建军，等。对……的研究[5]李建军，李建军，李建军，等。抗菌素耐药性模式及其编码基因…[6]张晓明，王晓明，王晓明，等。云南省云南省不同类型鲍曼不动杆菌的耐药特征及耐药性分析[j]。[7]李建军，李建军，李建军，等。中国烟草烟草行业的发展趋势。[8]王晓明，王晓明，王晓明，等。耐药分子分析…[9] García-Patiño王志强，García-Contreras R, Licona-Limón P.鲍曼不动杆菌的免疫应答，[j]。[10]李建军，李建军，李建军，等。河流中不动杆菌的耐药性研究…[11]张建军，张建军，张建军，等。鲍曼不动杆菌blaTEM基因的克隆及耐药性分析[j]。[12]张建军，张建军，张建军。的分子研究……[13]张建军，张建军，张建军，等。金属β-内酰胺酶的研究进展[j]。[14]王晓明，王晓明，王晓明，等。[15]李建军，李建军，李建军，等。抗菌……[16]李建军，李建军，李建军，等。出现……[17]李建军，李建军，李建军，等。[18]张晓明，张晓明，张晓明，等。抗菌药物的研究进展。[19]张建军，张建军，张建军，等。[20]李建军，李建军，李建军，等。[21]张志强，李志强。在硅检测…[22]李建军，李建军，李建军，等。流行的……[23]王晓明，王晓明，王晓明，等。[24]王晓明，王晓明，王晓明，等。中国小麦blaPER、blaVEB、blaCTX-M、tetA和tetB基因多态性分析。[25]张建军，张建军，张建军，等。[26]张建军，张建军，张建军，等。流行的……目的:鲍曼不动杆菌是一种条件致病菌，对包括β -内酰胺在内的许多抗生素具有耐药性。β-内酰胺酶的产生是鲍曼不动杆菌产生β-内酰胺耐药的主要机制。本研究的目的是确定临床分离的鲍曼不动杆菌中blabem和blaVEB基因的频率，以及在伊斯法罕烧伤创面感染分离的菌株中ESBL基因的存在与抗生素耐药性的关系。材料与方法:本研究从烧伤创面感染中分离出123株耐多药鲍曼不动杆菌。采用Kirby-Bauer圆盘扩散法进行抗生素耐药性评估后，采用聚合酶链反应(PCR)技术检测ESBL基因，最后进行统计学分析。结果:123株鲍曼不动杆菌PCR结果显示，77株(62.60%)为ESBL阳性。blaTEM和blaVEB基因的频率分别为52个(42.3%)和67个(54.5%)。鲍曼不动杆菌对抗生素的耐药性与ESBL基因(blaTEM和blaVEB)的存在有显著关系。结论:本研究发现的鲍曼不动杆菌株中blabem和blaVEB基因的高流行率是伊斯法罕和伊朗地区烧伤创面感染的主要问题，因为这些菌株引起的感染治疗非常复杂。本研究结果强调需要采取感染控制措施，以防止耐药分离株和ESBL基因的传播，特别是在烧伤医院。

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