Regeneration of Medicago arborea L. plants from tissue and protoplast cultures of different organ origin

Abstract

Remarkable yields of highly dividing protoplasts were isolated from rootlets (60-h-old) and mesophyll of Medicago arborea L. Plating efficiency was satisfactory for root and excellent for leaf protoplasts. Culture conditions were established for callus induction and growth from various organ explants and plant regeneration. Fertile plants were obtained from tissue and protoplast-derived calli through meristematic organogenesis. The results have been discussed in relation to somatic interspecific hybridization within the genus Medicago.