Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters

Abstract

Improving sperm parameters with a role in fertilization and keeping them as long as possible at optimal levels is still a current requirement for frozen-thawed semen. The aim of the study was to evaluate the effect of the addition of an artificial insemination media (M) and prostatic fluid (PF), post thawing, on dog sperm motility parameters. The sperm rich fraction from nine dog ejaculates was cryopreserved. After thawing, three experimental variants were carried out: 1) semen diluted 1:1 with CaniPlus Enhance–media 2) with the PF and 3) semen without any addition. Samples were incubated at 37°C and sperm motility parameters were assessed after 10, 25, 40 and 50 minutes. Samples supplemented with the insemination media recorded the highest values of total motility, progressive motility and sperm velocity throughout incubation. Addition of PF had a negative effect on the total and progressive motility values and caused a slight increase in velocity.