Evaluation of the secondary structure of poly-γ-glutamic acid produced by Bacillus subtilis EGP5QL12 by circular dichroism spectroscopy method

Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology Pub Date : 2021-09-24 DOI:10.18500/1816-9775-2021-21-3-274-279

M. V. Chernykh, I. M. Ibrahim, V. Grinev, E. Sigida, Y. P. Fedonenko, S. Konnova

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Abstract

An extracellular polymer was isolated from the culture liquid of Bacillus subtilis EGP5QL12 with the yield of 5,6 g/L. On the basis of the data of thin layer chromatography, colorimetric analyses and FTIR spectroscopy, it was established that the polymer is poly-γ- glutamic acid (PGA). PGA is widely used in medicine, cosmetology and the food industry due to its ability to bind water and metal ions. To assess the biotechnological potential of the isolated polymer and predict the possibilities of its application in various fields of the national economy, it is necessary to analyze the characteristic spectral features that make it possible to establish its secondary structure. The isolated PGA preparation was analyzed by circular dichroism spectroscopy at various pH values. According to the results of this study, it was found that the polymer forms predominantly β-structures with a low proportion of irregular structures and α-helices, which gives it a high potential for creating hydrogels and composite materials.

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圆二色光谱法评价枯草芽孢杆菌EGP5QL12产聚γ-谷氨酸的二级结构

从枯草芽孢杆菌EGP5QL12培养液中分离出一种胞外聚合物，产率为5.6 g/L。通过薄层色谱、比色分析和红外光谱分析，确定该聚合物为聚γ-谷氨酸(PGA)。PGA由于其结合水和金属离子的能力而广泛应用于医药、美容和食品工业。为了评价分离聚合物的生物技术潜力，预测其在国民经济各个领域的应用可能性，有必要对其特征光谱特征进行分析，使其二级结构的建立成为可能。用圆二色光谱分析了不同pH值下分离得到的PGA。根据本研究的结果，聚合物以β-结构为主，不规则结构和α-螺旋结构比例低，这使得它具有很高的水凝胶和复合材料的潜力。

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Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology

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