INDICATORS OF THE CARDIOVASCULAR SYSTEM OF RATS UNDER THE ACTION OF ACETYLSALICYLIC ACID AND ITS COMPLEX COMPOUNDS WITH METALS

Abstract

The effect of acetylsalicylic acid (ASA) and its complex compounds with the metals lithium (Li+), potassium (K+), magnesium (Mg2+) and calcium (Ca2+) with a single intraperitoneal injection at doses of 5, 10 and 20 mg/kg on cardiovascular system (CVS) of rats (blood pressure, heart rate, indicators of heart electrocardiogram). The structure-effect analysis showed that the coordination compounds of ASA, in which, in addition to the ligand with bioactive properties, a metal-microelement is present, caused multidirectional changes in the CVS indices. Those changes depended both on the metal included in the compound and on the dose of the administered substance. The study was carried out on the basis of the Center for collective use of scientific equipment «Experimental Physiology and Biophysics» (Department of Human and Animal Physiology and Biophysics, V.I. Vernadsky Crimean Federal University). The research of the biological effect of the acetylsalicylic acid, SLi+, SK+, SMg2+, SCa2+ was carried out during their intraperitoneal injection into rats in doses of 5, 10 and 20 mg/kg. Simultaneously the following characteristics of all the groups’ animals were registered: heart rate (HR), systolic blood pressure (SBP) and diastolic blood pressure (DBP) and electrocardiogram indicators. The pulse pressure (PP) was calculated on the basis of the SBP and DBP indices difference. BP, HR and RR of the rats were registered with the help of the system NIBP200A («Biopac Systems, Inc.», USA). The experimental data obtained in our studies confirm the literature data, which show that in the process of complexation there is not only an increase or decrease in certain effects that are characteristic of precursor molecules-salicylates, but also the appearance of new properties of derived substances. Тhe obtained data confirm the cardiotropic effectiveness of new coordination compounds, show the dose dependence of these effects and open up the prospects for further studies of their biological action when used repeatedly in chronic experiments.