Development of a New Nontoxic Thiazole Derivative Dye for Cell Labeling by Using Nanoparticles

Abstract

Fluorescent labeling of L-929 fibroblast cells was studied by using newly synthesized ligand which was named as 2- N -methylamino-5-(pyrene-1-yl)-thiazole (ABS 40). The maximum fluorescence intensity of ABS 40 was detected at an excitation and emission wavelengths of 350 nm and 400 nm. Fluorescence intensity of the ligand was changed by the addition of DNA from calf thymus (CT-DNA) and human serum albumin (HSA). Both biomolecules and ABS 40 formed complexes that changed the fluorescence intensity of the ligand at the given wavelengths. Cytotoxicity tests were done with XTT analysis. L-929 fibroblast cells were cultured in the presence of ABS 40 to show cell labeling. Results showed that ABS 40 at 318 μM was labeled the cells at an excitation wavelength of 403.7 nm by using confocal microscopy. in vivo cell labeling was also confirmed by using Ct-DNA immobilized iron nanoparticles.