Caracterización y evaluación de colorantes de Monnina vargassi y Vaccinium floribundum por espectroscopia infrarroja FTIR-ATR y UV Visible

Revista ECIPeru Pub Date : 2022-09-01 DOI:10.33017/reveciperu2022.0013/

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Abstract

Polyphenols and anthocyanins have been determined in fresh native fruits of Vaccinium floribundum and Monnina vargassi, for which their pigments were extracted with 0.01% acidified methanol, filtered, concentrated, centrifuged, decanted and scored to a defined volume for later evaluation.The anthocyanin spectra were identified by FTIR-ATR and Visible UV spectroscopy and the content of total anthocyanins (CAT) was evaluated by the differential pH method, total polyphenols (PFT) by the Folin-Ciocalteu method, found in the Visible and UV spectra. FTIR - ATR strong absorption signals at different values of vibration frequencies (cm-1) that correspond to functional groups of anthocyanins. The highest value in CAT corresponds to the extract of alaybili and the lowest to the condorusa with 108.12 and 56.07 (mg of cyanidin 3-glucoside / 100 g of fresh weight) respectively. Similarly, the highest value in PFT corresponds to alaybili and the lowest to condorusa with 627.13 and 342.24 (mg of gallic acid / 100 g of fresh weight) respectively. Therefore, it is concluded that the extracts of these fruits can be a source of functional foods as natural colorants in the food industry due to their high content of antioxidants. Keywords: Anthocyanins, Polyphenols, Antioxidants, Free Radicals, Flavonoids

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红外光谱FTIR-ATR和紫外可见光谱对蒙尼娜·瓦加西和花Vaccinium floribundum染料的表征与评价

采用0.01%酸化甲醇提取花莲和蒙地莲鲜果中的色素，经过滤、浓缩、离心、滗析并记分至规定体积，以测定其多酚和花青素含量。用FTIR-ATR和可见紫外光谱对花色苷进行鉴定，用差pH法测定总花色苷(CAT)的含量，用Folin-Ciocalteu法测定总多酚(PFT)的含量。FTIR - ATR强吸收信号在不同的振动频率值(cm-1)对应的花青素官能团。黄芪提取物的CAT值最高，分别为108.12和56.07 (mg / 100 g鲜重);同样，未食子酸的PFT值最高的是黄尾草，最低的是condorusa，分别为627.13和342.24 (mg / 100 g鲜重)。因此，这些水果的提取物可以作为食品工业的天然着色剂，因为它们含有高含量的抗氧化剂。关键词:花青素，多酚，抗氧化剂，自由基，类黄酮

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Revista ECIPeru

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