The influence of heating process on anticancer activity of Pterois volitans (red lionfish) venom extraction against human cervical carcinoma cell

THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019 Pub Date : 2019-12-10 DOI:10.1063/1.5139359

A. N. Sommeng, Mustika Sari, M. J. Ginting, M. Sahlan, H. Hermansyah, A. Wijanarko

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引用次数: 2

Abstract

Lionfish (Pterois volitans) is an invasive species originating from Indo-Pacific Sea. The rapid growth and the absence of natural predators cause Lionfish to breed rapidly and reduce the native fish populations by up to 80%. Therefore, the study of the Lionfish must be intensified to obtain the usefulness of these animals. Studies that have stated that the extract of poison lionfish spines has the potential to become an anticancer agent for cervical cancer. The aim of the present work is to Investigation the Anticancer activity of Pterois volitans from Indonesia to human cervical carcinoma cell (HeLa cell). Crude venom (CV) that is resulted from the extraction process by sonicating of fresh venomous Pterois volitans as a sample was used. The PLA 2 sample of crude venom was heated on (50, 55, 60, 65, 70) °C for 30 min and purified by (20, 40, 60, 80) % ammonium sulfate. The concentration and purity are analyzed using Lowry method, Marinette’s, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and ...

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加热过程对红狮鱼毒液提取物对人宫颈癌细胞抗癌活性的影响

狮子鱼(Pterois volitans)是一种源自印度太平洋的入侵物种。狮子鱼的快速生长和天敌的缺乏导致狮子鱼迅速繁殖，使本地鱼类的数量减少了80%。因此，必须加强对狮子鱼的研究，以获得这些动物的有用性。研究表明，毒狮子鱼刺的提取物有可能成为宫颈癌的抗癌剂。本研究旨在探讨印尼凤头龙葵对人宫颈癌细胞(HeLa细胞)的抗癌作用。以新鲜的毒翼龙胆为样品，通过超声提取得到的粗毒液(CV)为研究对象。在(50、55、60、65、70)℃加热30 min，用(20、40、60、80)%硫酸铵纯化。采用Lowry法、Marinette法、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对其浓度和纯度进行了分析。

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THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019

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