The effect of iodine excess on bone metabolism in experimental autoimmune thyroiditis rats

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-03-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.02.002

Z. Xin, Chen Ke-jun, Sang Zhongna, Zhao Na, Zhang Jin-xiang, Yao Zhai-xiao, Zhang Wan-qi, Ma Xin-long

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Abstract

Objective To explore the effect of iodine excess on bone metabolism in experimental autoimmune thyroiditis (EAT) rats. Methods We selected 36 female Lewis rats with body weight of (131 ± 15)g,and divided them into 3 groups randomly: control group, EAT group and EAT + high iodine group, assuring 12 rats in every group. These rats were fed fodder with different concentration of iodine(0.9,0.9, 18.0 mg/kg), and rats in EAT group and EAT + high iodine group were immunized with pig thyroglobulin(pTG) and complete Freund's adjuvant(CFA) to create EAT model. After two weeks, the pathological changes of the thyroid tissues were observed,and the serum thyroid autoantibody[thyroglobulin antibody(TGAb) and thyroid microsomal antibody(TMAb)], the thyroid hormone levels[triiodo thyronine(T3) and thyrine(T4)] and some relevant data of bone metabolism[bone gla protein (BGP), tartrate-resistant acid phosphatase (TRAP), C-terminal propeptide of type Ⅰ procollagen (PICP),C-terminal telopeptide of type Ⅰ collagen (ICTP), insulin-like growth factor- 1 ( IGF- 1 ), osteoprotegerin (OPG) and receptor activator of NF-κB ligand (RANKL)] were measured. Results Inflammatory cell infiltration and local follicular structural damage were observed in the thyroid tissues of EAT rats in EAT group and EAT + high iodine group, and the pathological changes of EAT + high iodine group were mainly thyroid follicular expansion and integration. The level of serum TGAb, TMAb, T3 and T4 of EAT rats in EAT group and EAT + high iodine group[ (63.01 ± 12.36)%, (60.62 ± 11.24)%, (3.78 ± 1.43), (125.12 ± 16.00)pmol/L and (75.00 ± 15.44)%,(72.15 ± 15.00)%, (3.69 ± 0.91 ), (149.40 ± 20.67)pmol/L] were higher than those of the control group[ (4.47 ±1.04)%, (5.73 ± 1.01 )%, (0.75 ± 0.12), (76.91 ± 9.30)pmol/L, all P ＜ 0.05], and the level of serum TGAb,TMAb and T4 of EAT rats in EAT + high iodine group were higher than those of the EAT group(all P ＜ 0.05).The level of serum BGP, PICP and IGF- 1 in EAT group[ ( 1.70 ± 0.31 ), ( 11.31 ± 1.52) μg/L, (0.31 ± 0.06 ) mg/L]were lower than those of the control group[ (8.60 ± 0.33), (14.28 ± 3.10)μg/L, (1.16 ± 0.02)mg/L, all P ＜0.05], and the level of serum TRAP, ICTP, OPG and RANKL[ ( 19.88 ± 3.60)ng/L, (2.43 ± 0.82), (22.36 ± 2.80),( 1.35 ± 0.23 )μg/L] were higher than those of the control group[ ( 14.57 ± 3.56)ng/L, (0.50 ± 0.20), (1.61 ± 0.34),(0.10 ± 0.02)μg/L, all P ＜ 0.05]; compared with EAT group, the level of PCIP and OPG in EAT + high iodine group [ (8.03 ± 1.84), ( 16.80 ± 3.79)μg/L] were obviously decreased(all P ＜ 0.05). Conclusions The reinforcement of differentiation and maturation of osteoblast in the EAT rats results in the increasing of bone resorption. The activity of osteoblast and osteoclast of the EAT rats are inhibited by excessive iodine, showing a low conversion-type osteoporosis. Key words: Iodine; Thyroiditis, autoimmune; Bone metabolism; Osteoblasts; Osteoclasts

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碘过量对实验性自身免疫性甲状腺炎大鼠骨代谢的影响

目的探讨碘过量对实验性自身免疫性甲状腺炎(EAT)大鼠骨代谢的影响。方法选取体重为(131±15)g的雌性Lewis大鼠36只，随机分为对照组、EAT组和EAT +高碘组，每组12只。分别饲喂不同浓度碘(0.9、0.9、18.0 mg/kg)的饲料，EAT组和EAT +高碘组分别免疫猪甲状腺球蛋白(pTG)和完全弗氏佐剂(CFA)，建立EAT模型。两周后观察甲状腺组织病理变化，血清甲状腺自身抗体[甲状腺球蛋白抗体(TGAb)和甲状腺微粒体抗体(TMAb)]、甲状腺激素水平[三碘甲状腺原氨酸(T3)和甲状腺素(T4)]及骨代谢相关数据[骨玻璃蛋白(BGP)、抗酒石酸酸性磷酸酶(TRAP)、Ⅰ型前胶原c端前肽(PICP)、Ⅰ型胶原c端端肽(ICTP)、测定胰岛素样生长因子- 1 (IGF- 1)、骨保护素(OPG)和NF-κ b配体受体激活剂(RANKL)。结果EAT组和EAT +高碘组大鼠甲状腺组织均可见炎性细胞浸润和局部滤泡结构损伤，EAT +高碘组的病理变化主要为甲状腺滤泡扩张和整合。EAT组和EAT +高碘组大鼠血清TGAb、TMAb、T3、T4水平[(63.01±12.36)%、(60.62±11.24)%、(3.78±1.43)、(125.12±16.00)pmol/L和(75.00±15.44)%、(72.15±15.00)%、(3.69±0.91)、(149.40±20.67)pmol/L]均高于对照组[(4.47±1.04)%、(5.73±1.01)%、(0.75±0.12)、(76.91±9.30)pmol/L，均P < 0.05];EAT +高碘组大鼠血清TGAb、TMAb、T4水平均高于EAT组(均P < 0.05)。血清水平边界网关协议,PICP和IGF - 1在吃组[(1.70±0.31),(11.31±1.52)μg / L,(0.31±0.06)mg / L)低于对照组((8.60±0.33),(14.28±3.10)μg / L,(1.16±0.02)mg / L,所有P < 0.05),血清水平的陷阱,ICTP,功能和RANKL (ng / L(19.88±3.60),(2.43±0.82),(22.36±2.80),(1.35±0.23)μg / L)高于对照组(ng / L(14.57±3.56),(0.50±0.20),(1.61±0.34),(0.10±0.02)μg / L,所有P < 0.05);与EAT组比较，EAT +高碘组PCIP、OPG水平[(8.03±1.84)、(16.80±3.79)μg/L]明显降低(均P < 0.05)。结论EAT大鼠成骨细胞分化和成熟的增强导致骨吸收的增加。过量碘抑制EAT大鼠成骨细胞和破骨细胞活性，表现为低转化型骨质疏松。关键词:碘;自身免疫性甲状腺炎;骨代谢;成骨细胞;破骨细胞

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.