Dynamic Adsorption Behaviors of Protein on Cibacron Blue-Modified PVA Nanofiber Fabrics

Q4 Materials Science

Journal of Textile Engineering Pub Date : 2021-02-15 DOI:10.4188/JTE.67.1

Song Liu, Y. Mukai

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引用次数: 3

Abstract

Electrospun polyvinyl alcohol (PVA) nanofiber fabrics functionalized by Cibacron Blue F3GA (CB) as an affinity ligand were prepared as efficient platforms for protein adsorption. Bovine serum albumin (BSA) was selected as a model protein to investigate their static adsorption behaviors. The protein adsorption capacities for the PVA nanofiber are 355.9 and 793.7 mg/g before and after CB modification, resulting in a 2.2 times increase. Then, dynamic experiments were conducted to determine the function of CB modification to the PVA nanofiber fabric. The effects of initial concentration and permeation rate on the dynamic adsorption behaviors for BSA of the CB-modified PVA nanofiber fabrics were also studied. The pseudo-first-order and pseudo-second-order kinetic models were used to analyze the kinetic adsorption data, and the latter was better fitted the experimental data. Furthermore, the adsorbed BSA can be easily eluted by a 0.1 M NaCl solution, and the CB-modified PVA nanofiber fabrics presented competent adsorption performance in the three-cycle reused experiment. Finally, the adsorption efficiency by the static and dynamic methods was compared. The obtained results demonstrate the potential of using the CB-modified PVA nanofiber for the affinity adsorption and isolation of proteins. and

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改性聚乙烯醇纳米纤维织物对蛋白质的动态吸附行为

以Cibacron Blue F3GA (CB)为亲和配体，制备了电纺丝聚乙烯醇(PVA)纳米纤维织物，作为蛋白质吸附的高效平台。以牛血清白蛋白(BSA)为模型蛋白，研究其静态吸附行为。改性前后PVA纳米纤维的蛋白质吸附量分别为355.9和793.7 mg/g，提高了2.2倍。在此基础上，通过动态实验确定了CB改性对PVA纳米纤维织物的作用。研究了初始浓度和渗透速率对cb改性PVA纳米纤维织物对牛血清白蛋白动态吸附行为的影响。采用拟一阶和拟二阶动力学模型对吸附动力学数据进行了分析，后者与实验数据拟合较好。此外，吸附的BSA可以很容易地被0.1 M NaCl溶液洗脱，并且在三循环重复使用实验中，cb改性PVA纳米纤维织物具有良好的吸附性能。最后比较了静态和动态两种方法的吸附效率。研究结果表明，利用cb修饰的聚乙烯醇纳米纤维对蛋白质进行亲和吸附和分离是有潜力的。和

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来源期刊

Journal of Textile Engineering Materials Science-Materials Science (all)

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： Journal of Textile Engineering (JTE) is a peer-reviewed, bimonthly journal in English and Japanese that includes articles related to science and technology in the textile and textile machinery fields. It publishes research works with originality in textile fields and receives high reputation for contributing to the advancement of textile science and also to the innovation of textile technology.