Growth Rate and Histamine Production of Klebsiella sp. CK02 Isolated from Skipjack Tuna Compared with Morganella morganii ATCC 25830 at Various Incubation Temperatures

Q3 Environmental Science
Aldino Dityanawarman, Indun Puspita Dewi, S. Ratnawati, N. Ekantari, M. Tamplin
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引用次数: 4

Abstract

One of an important quality parameter in tuna is the level of histamine content. The contamination of histamine in tuna is mainly due to the activity of histidine decarboxylase produced by the bacteria. A rapid growth of histamine producing bacteria is correlated with the practice of temperature abuse during handling. This study aimed to develop predictive growth modeling of two histamine-producing bacteria in the function of temperature. The growth and histamine production of Klebsiella sp. CK02 and Morganella morganii ATCC 25830 at various temperatures were measured in tryptic soy broth histidine (TSBH) and tuna fish infusion broth (TFIB) growth media. Broths were incubated at 4°C and 15°C for 7 days, and at 30°C and 40°C for 24 hours. The Baranyi and Roberts model was used with DMFit to determine primary growth kinectics, and the Ratkowsky square root model to describe bacterial growth rate as a function of temperature. Histamine production was enumerated by the apparent yield factor (pYhis/CFU) value. Growth rate increased with temperature, with a maximum rate at 40°C for Klebsiella sp. CK02 (0.740 log CFU/h) and M. morganii (0.578  log CFU/h). The Tmin for Klebsiella sp. CK02 in TFIB was -8.9°C, indicating better survival in low storage temperature, compare to M. morganii ATCC 25830. In addition, Klebsiella sp. CK02 produced a lower pYhis/CFU at 15 and 30°C compared to M. morganii ATCC 25830.
不同培养温度下鲣鱼克雷伯氏菌CK02与摩根摩根氏菌ATCC 25830的生长速率和组胺产量比较
金枪鱼的一项重要品质指标是组胺含量。金枪鱼中组胺的污染主要是由细菌产生的组氨酸脱羧酶活性引起的。产生组胺的细菌的快速生长与处理过程中滥用温度的做法有关。本研究旨在建立两种产组胺细菌在温度作用下的预测生长模型。测定了克雷伯氏菌CK02和摩根氏摩根氏菌ATCC 25830在胰蛋白酶豆汤组氨酸(TSBH)和金枪鱼汤汁(TFIB)培养基中不同温度下的生长和组胺产量。在4℃和15℃条件下孵育7天,在30℃和40℃条件下孵育24小时。使用Baranyi和Roberts模型与DMFit来确定初级生长动力学,并使用Ratkowsky平方根模型来描述细菌生长速率作为温度的函数。用表观产量因子(pYhis/CFU)值来列举组胺产量。生长速率随温度升高而升高,克雷伯氏菌CK02 (0.740 log CFU/h)和摩根氏芽孢杆菌(0.578 log CFU/h)在40℃时生长速率最大。克雷伯氏杆菌CK02在TFIB中的Tmin为-8.9°C,与M. morganii ATCC 25830相比,低温存贮存活率更高。此外,克雷伯氏菌sp. CK02在15°C和30°C时产生的pYhis/CFU比M. morganii ATCC 25830低。
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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