Isolation and identification of new beneficial bacterial strains from rhizosphere of Citrus sinensis orchards

Progress in Biological Sciences Pub Date : 2015-06-01 DOI:10.22059/PBS.2015.53957

M. Ramezani, A. R. Madvar, M. Khaleghi, R. Hemmati

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引用次数: 1

Abstract

The rhizosphere is the area around the root of a plant occupied by a unique population of usefulbacteria known as plant growth promoting rhizobacteria (PGPR). In this study, the isolation andidentification of rhizobacteria from orange (Citrus sinensis) orchards using 16S rRNA gene, as well asbiological and biochemical assays is reported. Analysis of 16S rRNA gene was confirmed bybiological and biochemical assays and showed that the isolated bacteria belong to the genera Bacilli,Enterobacter and Pseudomonas. Accordingly, Enterobacterkobei MR-R2, Pseudomonas putida MRR5,Bacillus cereus MR-R, Bacillus thuringiensis MR-R1 and Bacillus mycoides MR-R4 wereidentified and registered in GenBank. The results of phosphate solubilization testing revealed that themaximum rate of phosphate solubilization was observed for B. thuringiensis, and E. kobei; however,B. cereus, B. mycoides, and P. putida indicated the least activity. It can be concluded that the isolatedstrains (especially B. thuringiensis) are newly identified phosphate solubilizing bacterial strains,among which B. thuringiensis can be used for growth promotion of orange trees.

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柑桔根际有益菌的分离与鉴定

根际是植物根部周围的区域，由一种被称为促进植物生长的根瘤菌(PGPR)的独特有益细菌占据。本文报道了利用16S rRNA基因对柑橘根瘤菌进行分离鉴定，并对其进行了生物学和生化分析。经生物学和生化分析，鉴定分离的细菌属芽胞杆菌属、肠杆菌属和假单胞菌属。鉴定出kobei肠杆菌MR-R2、恶臭假单胞菌MRR5、蜡样芽孢杆菌MR-R、苏云金芽孢杆菌MR-R1、霉菌芽孢杆菌MR-R4，并在GenBank中登记。磷酸盐增溶试验结果表明，苏云金芽孢杆菌和神贝芽孢杆菌的磷酸盐增溶速率最大;然而,B。蜡样芽孢杆菌、真菌芽孢杆菌和恶臭杆菌活性最低。结果表明，分离得到的菌株(尤其是苏云金芽孢杆菌)是新鉴定的增磷菌，其中苏云金芽孢杆菌可用于橙树促生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Progress in Biological Sciences

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