Healing activities of Cramoll and xyloglucan membrane in cutaneous wounds of diabetic mice

Abstract

This study evaluated the healing potential of free Cramoll, a xyloglucan membrane extracted from Hymenaea courbaril (HcXM) and Cramoll immobilized in HcXM (Cramoll-HcXM), in cutaneous wounds of diabetic mice induced by alloxan. Sixty male Swiss albino mice were randomly divided into four experimental groups (15 animals): Control; Cramoll; HcXM; and Cramoll-HcXM. Wounds, on the animals’ back after anesthesia and trichotomy, were treated daily, and clinical analysis was performed daily. Microbiological, wound retraction, histological and collagen I and III quantification analyses were performed on the 2nd, 7th and 12th days of treatment. During the 12 days of treatment no contamination was observed to any experimental wounds. Reepithelialization was accelerated in Cramoll, HcXM and Cramoll-HcXM groups and wound retraction was statistically superior in the Cramoll group. The Cramoll and HcXM groups, after 12 days of treatment, presented epidermis and dermis with typical characteristics of healthy skin, with greater deposition of type 1 collagen. The treatment of cutaneous wounds in diabetic mice with Cramoll and HcXM accelerates the healing process when used individually, however, inflammatory infiltrate and fibrovascular tissue persisted in the Cramoll-HcXM group. According to the results, this is the first report on the use of a lectin as a healer in induced diabetic mice; Cramoll and HcXM are excellent compounds for repairing skin wounds in diabetics.